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Boston Biochem/Recombinant Human Ubiquitin Protein, CF/U-100H-10M

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Citations (72)

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SummaryProduct DatasheetsCarrier FreeReconstitution CalculatorBackgroundRelated Research Areas

Recombinant Human Ubiquitin Protein, CF Summary

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Activity

Recombinant Human Ubiquitin can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin concentration of 0.01-0.5 mM.

Source

E. coli-derived human Ubiquitin protein

Accession #

P0CG47

Predicted Molecular Mass

8.6 kDa

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

U-100H

Formulation	Lyophilized from a solution in deionized water.
Reconstitution	Reconstitute at 10 mg/mL in an aqueous solution.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Ubiquitin

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).

Highly purified Ubiquitin processed for the quantitative removal of glycine and buffer salts which can interfere with chemical and in vitro reactions.

References

Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
Wei, W. et al. (2004) Nature 428:194.
Wertz, I.E. et al. (2004) Nature 430:694.

Entrez Gene IDs

7314 (Human); 298693 (Rat)

Alternate Names

RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

Related Research Areas

Cell Structure and Signaling Molecules
Cell to Cell Adhesion Disassembly During EMT
HIF Transcription Factors
Negative Regulators of the Jak/STAT Pathway
Neurofibrillary Tangle Formation
NFkB Pathway
Notch Pathway
Parkinson"s Disease Related Molecules
Ubiquitin
Ubiquitin-related Molecules in the Akt Pathway
Wnt Intracellular Signaling

Citations for Recombinant Human Ubiquitin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.

72Citations: Showing 1 - 10Filter your results:

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Competitive ubiquitination activates the tumor suppressor p53Authors: X Li, M Guo, L Cai, T Du, Y Liu, HF Ding, H Wang, J Zhang, X Chen, C YanCell Death Differ., 2019;0(0):.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
Mitochondria ubiquitin ligase, MARCH5 resolves hepatitis B virus X protein aggregates in the liver pathogenesisAuthors: YS Yoo, YJ Park, HS Lee, NTK Oanh, MY Cho, J Heo, ES Lee, H Cho, YY Park, H ChoCell Death Dis, 2019;10(12):938.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15Authors: TC Ting, M Goralski, K Klein, B Wang, J Kim, Y Xie, D NijhawanCell Rep, 2019;29(6):1499-1510.e6.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
Human papillomavirus E7 oncoprotein targets RNF168 to hijack the host DNA damage responseAuthors: J Sitz, SA Blanchet, SF Gameiro, E Biquand, TM Morgan, M Galloy, J Dessapt, EG Lavoie, A Blondeau, BC Smith, JS Mymryk, CA Moody, A Fradet-TurProc. Natl. Acad. Sci. U.S.A., 2019;116(39):19552-19562.Species: Virus - HPVSample Types: Recombinant ProteinApplications: Bioassay
Uncoupling of p97 ATPase activity has a dominant negative effect on protein extractionAuthors: HB Rycenga, KB Wolfe, ES Yeh, DT LongSci Rep, 2019;9(1):10329.Species: XenopusSample Types: Egg ExtractApplications: Bioassay
Cbl interacts with multiple E2s in vitro and in cellsAuthors: MS Liyasova, K Ma, D Voeller, PE Ryan, J Chen, RE Klevit, S LipkowitzPLoS ONE, 2019;14(5):e0216967.Species: HumanSample Types: Recombinant ProteinApplications: Ubiquitination
K27-linked ubiquitination of BRAF by ITCH engages cytokine response to maintain MEK-ERK signalingAuthors: Q Yin, T Han, B Fang, G Zhang, C Zhang, ER Roberts, V Izumi, M Zheng, S Jiang, X Yin, M Kim, J Cai, EB Haura, JM Koomen, KSM Smalley, L WanNat Commun, 2019;10(1):1870.Species: HumanSample Types: Recombinant ProteinApplications: Ubiquitination
The FBXW7-SHOC2-Raptor Axis Controls the Cross-Talks between the RAS-ERK and mTORC1 Signaling PathwaysAuthors: CM Xie, M Tan, XT Lin, D Wu, Y Jiang, Y Tan, H Li, Y Ma, X Xiong, Y SunCell Rep, 2019;26(11):3037-3050.e4.Applications: Bioassay
DBC1 Regulates p53 Stability via Inhibition of CBP-Dependent p53 PolyubiquitinationAuthors: OE Akande, PK Damle, M Pop, NE Sherman, BB Szomju, LV Litovchick, SR GrossmanCell Rep, 2019;26(12):3323-3335.e4.Applications: Bioassay
SALL4 mediates teratogenicity as a thalidomide-dependent cereblon substrateAuthors: ME Matyskiela, S Couto, X Zheng, G Lu, J Hui, K Stamp, C Drew, Y Ren, M Wang, A Carpenter, CW Lee, T Clayton, W Fang, CC Lu, M Riley, P Abdubek, K Blease, J Hartke, G Kumar, R Vessey, M Rolfe, LG Hamann, PP ChamberlaiNat. Chem. Biol., 2018;0(0):.Species: HumanSample Types: Recombinant ProteinApplications: Ubiquitination

Discovery of Ubiquitin Deamidases in the Pathogenic Arsenal of Legionella pneumophilaAuthors: D Valleau, AT Quaile, H Cui, X Xu, E Evdokimova, C Chang, ME Cuff, ML Urbanus, S Houliston, CH Arrowsmith, AW Ensminger, A SavchenkoCell Rep, 2018;23(2):568-583.Applications: Bioassay

CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cellsAuthors: S Ito, A Ueno, T Ueda, H Nakagawa, H Taniguchi, N Kayukawa, A Fujihara-I, F Hongo, K Okihara, O UkimuraOncotarget, 2018;9(25):17645-17655.Applications: Bioassay

Age- and disease-dependent increase of the mitophagy marker phospho-ubiquitin in normal aging and Lewy body diseaseAuthors: X Hou, FC Fiesel, D Truban, M Castanedes, WL Lin, AI Soto, P Tacik, LG Rousseau, NN Diehl, MG Heckman, O Lorenzo-Be, I Ferrer, JM Arbelo, JC Steele, MJ Farrer, M Cornejo-Ol, L Torres, IF Mata, NR Graff-Radf, ZK Wszolek, OA Ross, ME Murray, DW Dickson, W SpringerAutophagy, 2018;14(8):1404-1418.Applications: Bioassay

Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndromeAuthors: KA Donovan, J An, RP Nowak, JC Yuan, EC Fink, BC Berry, BL Ebert, ES FischerElife, 2018;7(0):.Species: HumanSample Types: ProteinApplications: Ubiquitination

XIAP facilitates breast and colon carcinoma growth via promotion of p62 depletion through ubiquitination-dependent proteasomal degradationAuthors: X Huang, XN Wang, XD Yuan, WY Wu, PE Lobie, Z WuOncogene, 2018;0(0):.Species: HumanSample Types: ProteinApplications: Bioassay

Cryo-EM structures and dynamics of substrate-engaged human 26S proteasomeAuthors: Y Dong, S Zhang, Z Wu, X Li, WL Wang, Y Zhu, S Stoilova-M, Y Lu, D Finley, Y MaoNature, 2018;0(0):.Species: HumanSample Types: ProteinApplications: Bioassay

MDM2-mediated degradation of WRN promotes cellular senescence in a p53-independent mannerAuthors: B Liu, J Yi, X Yang, L Liu, X Lou, Z Zhang, H Qi, Z Wang, J Zou, WG Zhu, W Gu, J LuoOncogene, 2018;0(0):.Species: HumanSample Types: ProteinApplications: Bioassay

The E3 ubiquitin ligase Siah-1 suppresses avian reovirus infection by targeting p10 for degradationAuthors: X Chen, Z He, M Fu, Y Wang, H Wu, X Li, H Cao, SJ ZhengJ. Virol., 2018;0(0):.Applications: Bioassay

Inhibition of UVSSA ubiquitination suppresses transcription-coupled nucleotide excision repair deficiency caused by dissociation from USP7Authors: M Higa, K Tanaka, M SaijoFEBS J., 2018;0(0):.Applications: Bioassay

Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing FactorsAuthors: TR de Moura, S Mozaffari-, CZK Szabó, J Schmitzová, O Dybkov, C Cretu, M Kachala, D Svergun, H Urlaub, R Lührmann, V PenaMol. Cell, 2018;69(6):979-992.e6.Species: BacteriaSample Types: Cell LysatesApplications: Bioassay

Identification and functional characterization of arginine vasopressin receptor 1A : atypical chemokine receptor 3 heteromers in vascular smooth muscleAuthors: LJ Albee, HM LaPorte, X Gao, JM Eby, YH Cheng, AM Nevins, BF Volkman, V Gaponenko, M MajetschakOpen Biol, 2018;8(1):.Species: HumanSample Types: Whole CellsApplications: Bioassay

In vitro characterization of six STUB1 variants in spinocerebellar ataxia 16 reveals altered structural properties for the encoded CHIP proteinsAuthors: Y Pakdaman, M Sanchez-Gu, R Kleppe, S Erdal, HJ Bustad, L Bjørkhaug, K Haugarvoll, C Tzoulis, K Heimdal, PM Knappskog, S Johansson, I AukrustBiosci. Rep., 2017;37(2):.Species: HumanSample Types: ProteinApplications: Bioassay

Negative regulation of the RLH signaling by the E3 ubiquitin ligase RNF114Authors: B Lin, Q Ke, H Li, NS Pheifer, DC Velliquett, DW LeamanCytokine, 2017;0(0):.Species: HumanSample Types: Whole CellsApplications: Bioassay

In vitro analysis of RQC activities provides insights into the mechanism and function of CAT tailingAuthors: BA Osuna, CJ Howard, S Kc, A Frost, DE WeinbergElife, 2017;6(0):.Species: Yeast - Saccharomyces cerevisiaeSample Types: RNAApplications: Bioassay

An inhibitor of the proteasomal deubiquitinating enzyme USP14 induces tau elimination in cultured neuronsAuthors: M Boselli, BH Lee, J Robert, MA Prado, SW Min, C Cheng, MC Silva, C Seong, S Elsasser, KM Hatle, TC Gahman, SP Gygi, SJ Haggarty, L Gan, RW King, D FinleyJ. Biol. Chem., 2017;0(0):.Species: HumanSample Types: ProteinApplications: Enzyme Assay

Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosisAuthors: S Wang, J Juan, Z Zhang, Y Du, Y Xu, J Tong, B Cao, MF Moran, Y Zeng, X MaoCell Death Dis, 2017;8(9):e3058.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay

RNF8- and Ube2S-Dependent Ubiquitin Lysine 11-Linkage Modification in Response to DNA DamageAuthors: A Paul, B WangMol. Cell, 2017;66(4):458-472.e5.Applications: Bioassay

The Banana Fruit SINA Ubiquitin Ligase MaSINA1 Regulates the Stability of MaICE1 to be Negatively Involved in Cold Stress ResponseAuthors: ZQ Fan, JY Chen, JF Kuang, WJ Lu, W ShanFront Plant Sci, 2017;8(0):995.Applications: Bioassay

Distinct kinetics of serine and threonine dephosphorylation are essential for mitosisAuthors: JB Hein, EPT Hertz, DH Garvanska, T Kruse, J NilssonNat. Cell Biol., 2017;19(12):1433-1440.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay

Ubiquitination and regulation of AURKA identifies a hypoxia-independent E3 ligase activity of VHLAuthors: E Hasanov, G Chen, P Chowdhury, J Weldon, Z Ding, E Jonasch, S Sen, CL Walker, R DereOncogene, 2017;0(0):.Species: N/ASample Types: ProteinApplications: Ubiquitination

BAP1 regulates IP3R3-mediated Ca(2+) flux to mitochondria suppressing cell transformationAuthors: A Bononi, C Giorgi, S Patergnani, D Larson, K Verbruggen, M Tanji, L Pellegrini, V Signorato, F Olivetto, S Pastorino, M Nasu, A Napolitano, G Gaudino, P Morris, G Sakamoto, LK Ferris, A Danese, A Raimondi, C Tacchetti, S Kuchay, HI Pass, EB Affar, H Yang, P Pinton, M CarboneNature, 2017;546(7659):549-553.Species: N/ASample Types: ProteinApplications: Bioassay

Plk1 phosphorylation of CAP-H2 triggers chromosome condensation by condensin II at the early phase of mitosisAuthors: Y Kagami, M Ono, K YoshidaSci Rep, 2017;7(1):5583.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay

A20 regulates the DNA damage response and mediates tumor cell resistance to DNA damaging therapyAuthors: C Yang, W Zang, Z Tang, Y Ji, R Xu, Y Yang, A Luo, B Hu, Z Zhang, Z Liu, X ZhengCancer Res., 2017;0(0):.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay

Development of ?-Hairpin Peptides for the Measurement of SCF-Family E3 Ligase Activity in Vitro via Ornithine UbiquitinationAuthors: KM Houston, AT Melvin, GS Woss, EL Fayer, ML Waters, NL AllbrittonACS Omega, 2017;2(3):1198-1206.Species: HumanSample Types: ProteinApplications: Bioassay

pSILAC mass spectrometry reveals ZFP91 as IMiD-dependent substrate of the CRL4(CRBN) ubiquitin ligaseAuthors: J An, CM Ponthier, R Sack, J Seebacher, MB Stadler, KA Donovan, ES FischerNat Commun, 2017;8(0):15398.Species: N/ASample Types: ProteinApplications: Bioassay

The E3 ubiquitin ligase RNF185 facilitates the cGAS-mediated innate immune responseAuthors: Q Wang, L Huang, Z Hong, Z Lv, Z Mao, Y Tang, X Kong, S Li, Y Cui, H Liu, L Zhang, X Zhang, L Jiang, C Wang, Q ZhouPLoS Pathog, 2017;13(3):e1006264.Species: N/ASample Types: ProteinApplications: Bioassay

Prefoldins Negatively Regulate Cold Acclimation in Arabidopsis Thaliana By Promoting Nuclear Proteasome-Mediated HY5 DegradationAuthors: C Perea-Resa, MA Rodríguez-, E Iniesto, V Rubio, J SalinasMol Plant, 2017;0(0):.Applications: Bioassay

Molecular Mechanism of Substrate Processing by the Cdc48 ATPase ComplexAuthors: NO Bodnar, TA RapoportCell, 2017;169(4):722-735.e9.Applications: Bioassay

The Nedd8 Non-covalent Binding Region in the Smurf HECT Domain is Critical to its Ubiquitn Ligase FunctionAuthors: S He, Y Cao, P Xie, G Dong, L ZhangSci Rep, 2017;7(0):41364.Species: HumanSample Types: Cell LysatesApplications: Bioassay

H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesisAuthors: X Zhang, B Li, AH Rezaeian, X Xu, PC Chou, G Jin, F Han, BS Pan, CY Wang, J Long, A Zhang, CY Huang, FJ Tsai, CH Tsai, C Logothetis, HK LinNat Commun, 2017;8(0):14799.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay

RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous RecombinationAuthors: S Inano, K Sato, Y Katsuki, W Kobayashi, H Tanaka, K Nakajima, S Nakada, H Miyoshi, K Knies, A Takaori-Ko, D Schindler, M Ishiai, H Kurumizaka, M TakataMol. Cell, 2017;66(5):622-634.e8.Species: HumanSample Types: ProteinApplications: Enzyme Assay

DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residuesAuthors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L DubrezCell Death Dis, 2017;8(5):e2816.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay

HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancerAuthors: SY Kim, HJ Kim, HJ Kim, DH Kim, CH Kim, JH Han, HK Byeon, K LeeAutophagy, 2017;0(0):1-89.Species: HumanSample Types: Cell LysatesApplications: Bioassay

DNA damage and S phase-dependent E2F1 stabilization requires the cIAP1 E3-ubiquitin ligase and is associated with K63-poly-ubiquitination on lysine 161/164 residuesAuthors: V Glorian, J Allègre, J Berthelet, B Dumetier, PM Boutanquoi, N Droin, C Kayaci, J Cartier, S Gemble, G Marcion, D Gonzalez, R Boidot, C Garrido, O Michaud, E Solary, L DubrezCell Death Dis, 2017;8(5):e2816.Species: Bacteria - E. ColiSample Types: ProteinApplications: Bioassay

RNF11 sequestration of the E3 ligase SMURF2 on membranes antagonizes SMAD7 down-regulation of transforming growth factor ? signalingAuthors: RJ Malonis, W Fu, MJ Jelcic, M Thompson, BS Canter, M Tsikitis, FJ Esteva, I SánchezJ. Biol. Chem., 2017;292(18):7435-7451.Applications: Bioassay

FBXW7 Facilitates Nonhomologous End-Joining via K63-Linked Polyubiquitylation of XRCC4Authors: Q Zhang, D Karnak, M Tan, TS Lawrence, MA Morgan, Y SunMol. Cell, 2016;61(3):419-33.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay

Higher-order oligomerization promotes localization of SPOP to liquid nuclear specklesEMBO J, 2016;0(0):.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay

Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewalNat Commun, 2016;7(0):12326.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay

Neddylation E2 UBE2F promotes the survival of lung cancer cells by activating CRL5 to degrade NOXA via the K11 linkageClin Cancer Res, 2016;0(0):.Species: HumanSample Types: ProteinApplications: Ubiquitination

Regulation of the MDM2-p53 pathway by the nucleolar protein CSIG in response to nucleolar stressSci Rep, 2016;6(0):36171.Species: HumanSample Types: ProteinApplications: Bioassay

Structural insights into Parkin substrate lysine targeting from minimal Miro substratesSci Rep, 2016;6(0):33019.Species: HumanSample Types: ProteinApplications: Bioassay

TRIM65-catalized ubiquitination is essential for MDA5-mediated antiviral innate immunityAuthors: Xueting LangJ. Exp. Med, 2016;0(0):.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay

A novel cereblon modulator recruits GSPT1 to the CRL4(CRBN) ubiquitin ligaseAuthors: Mary E MatyskielaNature, 2016;0(0):.Species: N/ASample Types: ProteinApplications: Bioassay

Monoubiquitination Inhibits the Actin Bundling Activity of FascinAuthors: Shengyu YangJ. Biol. Chem., 2016;0(0):.Applications: Bioassay

An anti-ubiquitin antibody response in transitional cell carcinoma of the urinarybladder.Authors: Ardelt P, Ebbing J, Adams F, Reiss C, Arap W, Pasqualini R, Bachmann A, Wetterauer U, Riedmiller H, Kneitz BPLoS ONE, 2015;10(3):e0118646.Species: HumanSample Types: SerumApplications: ELISA

Ubiquitin-conjugating enzyme Cdc34 and ubiquitin ligase Skp1-cullin-F-box ligase (SCF) interact through multiple conformations.Authors: Sandoval D, Hill S, Ziemba A, Lewis S, Kuhlman B, Kleiger GJ Biol Chem, 2015;290(2):1106-18.Species: Bacteria - E. ColiSample Types: Whole Cells

Stochastic gate dynamics regulate the catalytic activity of ubiquitination enzymes.Authors: Rout, Manoj K, Hodge, Curtis D, Markin, Craig J, Xu, Xin, Glover, J N Mark, Xiao, Wei, Spyracopoulos, LeoJ Am Chem Soc, 2014;136(50):17446-58.Species: N/ASample Types: ProteinApplications: Bioassay

Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity.Authors: Guicciardi, Maria Eu, Werneburg, Nathan W, Bronk, Steven F, Franke, Adrian, Yagita, Hideo, Thomas, Gary, Gores, GregoryPLoS ONE, 2014;9(3):e92124.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay

The ubiquitin ligase ASB4 promotes trophoblast differentiation through the degradation of ID2.Authors: Townley-Tilson, W H Davi, Wu, Yaxu, Ferguson, James E, Patterson, CamPLoS ONE, 2014;9(2):e89451.Species: HumanSample Types: Whole CellsApplications: Ubiquitination

Pin1 modulates ERalpha levels in breast cancer through inhibition of phosphorylation-dependent ubiquitination and degradation.Authors: Rajbhandari P, Schalper K, Solodin N, Ellison-Zelski S, Ping Lu K, Rimm D, Alarid EOncogene, 2014;33(11):1438-47.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay

Dimeric Ube2g2 simultaneously engages donor and acceptor ubiquitins to form Lys48-linked ubiquitin chains.Authors: Liu, Weixiao, Shang, Yonglian, Zeng, Yan, Liu, Chao, Li, Yanchang, Zhai, Linhui, Wang, Pan, Lou, Jizhong, Xu, Ping, Ye, Yihong, Li, WeiEMBO J, 2014;33(1):46-61.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay

The spindle and kinetochore-associated (Ska) complex enhances binding of the anaphase-promoting complex/cyclosome (APC/C) to chromosomes and promotes mitotic exit.Authors: Sivakumar, Sushama, Daum, John R, Tipton, Aaron R, Rankin, Susannah, Gorbsky, Gary JMol Biol Cell, 2014;25(5):594-605.Species: HumanSample Types: Cell LysatesApplications: Bioassay

Ubiquitination increases parkin activity to promote autophagic alpha-synucleinclearance.Authors: Lonskaya I, Desforges N, Hebron M, Moussa CPLoS ONE, 2013;8(12):e83914.Species: N/ASample Types: Recombinant ProteinApplications: Enzyme Assay

CXC chemokine receptor 4 signaling upon co-activation with stromal cell-derivedfactor-1alpha and ubiquitin.Authors: Tripathi A, Davis J, Staren D, Volkman B, Majetschak MCytokine, 2013;65(2):121-5.Species: HumanSample Types: Whole CellsApplications: Bioassay

A pathogen type III effector with a novel E3 ubiquitin ligase architecture.Authors: Singer, Alexande, Schulze, Sebastia, Skarina, Tatiana, Xu, Xiaohui, Cui, Hong, Eschen-Lippold, Lennart, Egler, Monique, Srikumar, Tharan, Raught, Brian, Lee, Justin, Scheel, Dierk, Savchenko, Alexei, Bonas, UllaPLoS Pathog, 2013;9(1):e1003121.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay

Parkin ubiquitinates Tar-DNA binding protein-43 (TDP-43) and promotes its cytosolic accumulation via interaction with histone deacetylase 6 (HDAC6).Authors: Hebron, Michaeli, Lonskaya, Irina, Sharpe, Kaydee, Weerasinghe, Puwakdan, Algarzae, Norah K, Shekoyan, Ashot R, Moussa, CharbelJ Biol Chem, 2013;288(6):4103-15.Species: N/ASample Types: Recombinant ProteinApplications: Bioassay

p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO.Authors: Shibata Y, Oyama M, Kozuka-Hata H, Han X, Tanaka Y, Gohda J, Inoue JNat Commun, 2012;3(0):1061.Species: N/ASample Types: Recombinant ProteinApplications: Binding Assay

The APC/C subunit Mnd2/Apc15 promotes Cdc20 autoubiquitination and spindleassembly checkpoint inactivation.Authors: Foster S, Morgan DMol Cell, 2012;47(6):921-32.Species: N/ASample Types: ProteinApplications: Bioassay

Characterization of ML-IAP protein stability and physiological role in vivo.Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, DomagojBiochem J, 2012;447(3):427-36.Species: HumanSample Types: ProteinApplications: Bioassay

DNA damage-induced heterogeneous nuclear ribonucleoprotein K sumoylationregulates p53 transcriptional activation.Authors: Pelisch F, Pozzi B, Risso G, Munoz M, Srebrow AJ Biol Chem, 2012;287(36):30789-99.Species: N/ASample Types: ProteinApplications: Bioassay

ERalpha phosphorylation at Y537 by Src triggers E6-AP-ERalpha binding, ERalphaubiquitylation, promoter occupancy, and target gene expression.Authors: Sun J, Zhou W, Kaliappan K, Nawaz Z, Slingerland JMol Endocrinol, 2012;26(9):1567-77.Species: HumanSample Types: ProteinApplications: Bioassay

The F-box protein FBXO44 mediates BRCA1 ubiquitination and degradation.Authors: Lu, Yunzhe, Li, Jiezhi, Cheng, Dongmei, Parameswaran, Balaji, Zhang, Shaohua, Jiang, Zefei, Yew, P Renee, Peng, Junmin, Ye, Qinong, Hu, YanfenJ Biol Chem, 2012;287(49):41014-22.Species: N/ASample Types: Recombinant ProteinApplications: Ubiquitination

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Recombinant Human Ubiquitin Protein, CF

By Anonymouson 03/18/2019

Application: Enzymatic activity in vitroReason for Rating: Ubiquitin works great for auto-ubiquitination assays

Used in standard E1, E2, E3, ATP, Ubiquitin assays done in gel

Recombinant Human Ubiquitin Protein, CF

By Anonymouson 10/20/2017

Application: Enzymatic activity in vitro

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Boston Biochem/Recombinant Human Ubiquitin Protein, CF/U-100H-10M

Recombinant Human Ubiquitin Protein, CF Summary

Product Datasheets

Carrier Free

U-100H

Reconstitution Calculator

Background: Ubiquitin

Related Research Areas

Citations for Recombinant Human Ubiquitin Protein, CF

FAQs

Reconstitution Buffers

Reconstitution Buffer 1 (PBS)

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