产品资料

Immunoway/AF-4 Polyclonal Antibody/50ug 100ug/YT0140

AF-4 Polyclonal Antibody

Immunogen：
The antiserum was produced against synthesized peptide derived from human AF4. AA range:1-50

Dilution：
WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification：
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Other Name：
AFF1; AF4; FEL; MLLT2; PBM1; AF4/FMR2 family member 1; ALL1-fused gene from chromosome 4 protein; Protein AF-4; Protein FEL; Proto-oncogene AF4

Background：
AF4/FMR2 family member 1(AFF1)Homo sapiensThis gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in mu

Function：
disease:A chromosomal aberration involving AFF1 is associated with acute leukemias. Translocation t(4;11)(q21;q23) with MLL/HRX. The result is a rogue activator protein.,similarity:Belongs to the AF4 family.,

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using AF4 Antibody. The picture on the right is blocked with the synthesized peptide.