- Specifications
- Tech Info / Data
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- Documentation
Specifications
| Formulation | Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA) |
| Molecular Weight | 19.1 kDa |
| Source | cDNA obtained from mouse leukocyte interferon expressed in E.coli |
| Purity | > 95% by SDS-PAGE stained by Coomassie BlueEndotoxin level < 1 EU/μg |
| Bioactivity | Measured using a cytopathic inhibition assay on Mouse (L929) cells with EMCV.The EC50 for IFN in this assay is ~ 5 U/ml. |
| Storage | For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles |
| Synonyms | Mu IFN-α11, Mouse IFN Alpha Subtype, Mouse IFN Protein Subtype, Interferon Alpha, IFN Alpha, IFN-a, Type I IFN Alpha |
| Accession Number | AY225954 |
Tech Info / Data
Mouse IFN Alpha 11 was originally identified as an interferon whose expression was not upregulated by viral infection of cells [Coulombel et al. (1991) Gene 104(2):187]
Expression of Mouse IFN Alpha 11 is induced by poly(I):poly(C) and some virus, but not others in L929 cells [Fung et al. (2004) J. Imm. Meth. 284:177]. It has been suggested that expression of this IFN may be regulated by OCT-1 and Ptx-1 [Mesplede et al. (2005) Mol. Cell Biol. 25(19):8717, Lopez et al. Mol. Cell Biol. 10(20):7527]. Additionally, a number of studies have suggested that the expression of this IFN is tightly regulated [reviewed by Doly et al. (1998) Cell. Mol. Life Sci. 54:1109].
Summary: Mouse IFN Alpha 11 is a high activity interferon whose expression has been extensively studied and is tightly regulated.
For more information on Mouse IFN Alpha subtype nomenclature click here.
Citations
Documentation
12125-1 Certificate of Analysis (CoA) SDSComparison of Mouse IFN Alpha 11 and Mouse IFN Alpha A Antiviral Activity
The activity of Mouse IFN Alpha 11 and Mouse IFN Alpha A was compared. The EC50 for Mouse IFN Alpha 11 was 7 pg/ml.
Results are representative and may vary depending upon experimental conditions.