- Specifications
- Tech Info / Data
- Citations
- Documentation
Specifications
| Formulation | Phosphate-buffered saline (PBS) |
| Antigen | U266 Myeloma Cells |
| Isotype | Mouse IgG1, kappa |
| Host Species | Mouse |
| Purity | > 95% by SDS-PAGE stained by Coomassie BlueEndotoxin level < 1 EU/μg |
| Specificity | Detects human IFNAR1 |
| Storage | After receipt, store at -20oC for short-term use (≤6 months).For retention of full activity store at -70oC or below and avoid repeated freeze/thaw cycles |
| Clone | MMHAR-3 |
Tech Info / Data
Protocols and Guides:
- General Procedure for Cell Surface Staining using 21370-1: Mouse MAb to Human IFN Alpha/Beta Receptor 1 (link)
Tested Applications:
- Direct Binding ELISA
- Flow Cytometry
Citations
Documentation
21370-1 Certificate of Analysis (CoA) Product Flyer SDSRepresentative Binding Curves (ELISA)
Representative binding curve of antibody to mammalian expressed human interferon alpha/beta R1 protein and to 1% BSA/PBS in a Direct Binding ELISA. High-binding polystyrene plates were coated with either 1 μg/ml of human interferon alpha/beta R1 protein or 1% BSA/PBS. Thereafter, titrations of the product were added to the wells. Goat anti-mouse IgG conjugated to HRP was used as the detection antibody. Colorimetric detection was performed using 3,3",5,5"-Tetramethylbenzidine (TMB) substrate. The HRP-TMB reaction was stopped using a diluted H2SO4/HCl solution.