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BD Biosciences/Control γ<sub>1</sub> FITC/γ<sub>1</sub> PE/CD45 PerCP/340385188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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BD Biosciences/Control γ1 FITC/γ1 PE/CD45 PerCP/340385

Product DetailsRecommended AssayReferences

Description

Becton Dickinson Immunocytometry Systems (BDIS) Tritest ™ Control, γ1 fluorescein isothiocyanate (FITC)/γ1 phycoerythrin (PE)/CD45 peridinin chlorophyll (PerCP), is a three-color direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with BD Tritest reagents on the Becton Dickinson family of flow cytometers. The Tritest Control is used to determine the unstained lymphocyte population on a fluorescence 1 (FL1) vs fluorescence 2 (FL2) display and to determine any nonantigen-specific antibody binding (nonspecific staining) present, particularly that caused by Fc receptors.

BD Tritest Control is for in vitro diagnostic use with BD in vitro diagnostic Tritest reagents.

Contents

SpecificityCloneFormatIsotypeEntrez Gene ID
X40FITCIgG1, κ3811
X40PEIgG1, κ3811
CD452D1PerCPIgG1, κ
Preparation and Storage

1. For in vitro diagnostic use.

2. Store the reagent at 2° to 8°C to maintain stability. Do not use after expiration date shown on the label.

3. Do not freeze the reagent or expose it to direct light during storage or during incubation with cells. Keep the reagent vial dry.

4. Do not use the reagent if you observe any changes in appearance. Precipitation or discoloration indicates instability or deterioration.

5. The antibody reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which may cause erroneous results.

  1. Enumeration of Immunologically Defined Cell Populations by Flow Cytometry; Approved Standard—Second Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2007. CLSI document H42-A2.

  2. Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture ; Approved Standard— Sixth Edition. Wayne, PA: Clinical and Laboratory Standards Institute, 2007. CLSI document GP41-A6.

  3. Protection of Laboratory Workers from Occupationally Acquired Infections; Approved guideline—Third Edition. Wayne, PA: Clinical and Laboratory Standards Institute, 2005. CLSI document M29-A3.

  4. Centers for Disease Control. 1997 Revised guidelines for the performance of the CD4+ T-cell determinations in persons with human immunodeficiency virus (HIV) infection. In: MMWR. 1997;46:1-29

  5. Centers for Disease Control. Perspectives in disease prevention and health promotion update: Universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens on health-care settings. MMWR. 1988;37:377-388.

  6. Cobbold S, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael A, ed. Leucocyte Typing III. Oxford: Oxford University Press. 1987;1:797.

  7. Giorgi J. Lymphocyte subset measurements: Significance in clinical medicine. In: Rose N, Friedman H, Fahey J, ed. Manual of Clinical Laboratory Standards. Washington, DC: American Society for Microbiology; 1986:236-246.

  8. Jackson A, Warner N. Preparation, staining, and analysis by flow cytometry of peripheral blood leukocytes. In: Rose N, Friedman H, Fahey J, eds. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:226-235.

  9. Nicholson J, Browning S, Orloff S, McDougal J. Inactivation of HIV-infected H9 cells in whole blood preparations by lysing/fixing reagents used in flow cytometry. J Immunol Methods. 1993;160:215-218.

  10. Nicholson J, Jones B, Hubbard M. CD4 T-lymphocyte determinations on whole blood specimens using a single-tube three-color assay. Cytometry. 1993;14(August):598-605.

  11. Schwinzer R. Cluster report: CD45/CD45R. In: Knapp W, Dörken B, Gilks W, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press Inc; 1989:628-634.

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