High Sensitivity: Lower Ct values ensure higher efficiency of amplificationCost Effective: Offered at much discounted price compared to market price
Bimake™ 2x SYBR Green qPCR master mix (Low ROX) utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR. The mix with low concentration of ROX, prepared at 2x reaction concentration, can be directly used for robust and low-template quantitative PCR on Applied Biosystems 7500, 7500 Fast, ViiA™7; Stratagene MX4000™, MX3005P™, MX3000P™.
Dye-based quantitative PCR detection Nucleic acid amplification and expression profilingQuantitative genotyping studiesGenomics-related applications
Component | Cat#: B21702 | Cat#: B21703 |
---|---|---|
Bimake™ 2x SYBR Green qPCR Master Mix※(Low ROX) | 5 mL (for 200 reactions with 50 μL/rxn for 500 reactions with 20 μL/rxn for 1000 reactions with 10 μL/rxn) | 25 mL (for 1000 reactions with 50 μL/rxn for 2500 reactions with 20 μL/rxn for 5000 reactions with 10 μL/rxn) |
※ Contains hot-start DNA Polymerase, dNTPs, Mg2+, SYBR Green I dye and ROX Dye (Low concentration) |
Store at -20°C for up to 2 years. Repetitive freeze-thaw cycles should be avoided. It is recommended to pre-aliquot the mix into small batches for frequent usage. Product quality is guaranteed under proper storage conditions.
User Guide | 2x SYBR Green qPCR Master Mix (Low ROX)
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