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| Description | 3-D Life SG-Dextran is a component of the 3-D Life Hydrogel system. Itcontains dextran functionalized with thiol-reactive groups (SG-Dextran) thatcan be used together with PEG-Link (Cat. No.L50-1 or L50-3) or CD-Link (Cat.No. L60-1 or L60-3) to set up slow gelling, cell-compatible hydrogels. Dextran-based 3-D Life hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recoveryof chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or forfurther cultivation. 10 x CB (pH 7.2) is a buffer to control pH and osmotic conditions during gelformation in the presence of cells. For more information and instructions,please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide. | ||||||||||||||||||||
| Application | 3-D cell cultures; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution. | ||||||||||||||||||||
| Quantity | Allows formation of up to 6 ml 3-D Life Hydrogel depending on the stiffness of the gel, if used together with thiol-functionalized polymers (3-D Life PEG-Link or CD-Link). | ||||||||||||||||||||
| Components |
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| Detailed Product Information |
| Slow-gelling hydrogels |
| Cellendes user guide |
| Degradation of dextran-based hydrogels with dextranase. |
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