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CohesionBio/hsa-let-7b-5p miRNA Mimic/5 nmol/CMH0002

Description Synthetic miRNA mimics are used to overexpress hsa-let-7b-5p by transfaction.
Specificity miRNA Mimics are chemically modified; double-stranded miRNA-like RNA which are designed to copy the functionality of mature endogenous miRNA upon transfection. At the 5"-end; it is synthesized with a partially complementary motif to 3"UTR end of the target gene; which allows the miRNA mimic to specifically bind to the target. Transfection of mimics followed by downstream gene expression analysis or phenotypic analysis; is performed to elucidate the targets and roles of particular miRNAs.
Form Lyophilized powder
Gene Symbol hsa-let-7b-5p
Entrez Gene (Human) MIMAT0000063
Directions for Use We recommend re-suspending the lyophilized synthetic miRNA using DNase and RNase-free ddH2O. To make a 100 uM stock; dissolve the lyophilized powder using 50 ul of ddH2O.
Components This synthetic miRNA is based on the mature miRNA sequence. It does not contain the full precursor miRNA stem-loop.
Storage/Stability Shipped at 4 deg C. Store at -20 deg C for one year. Avoid freeze-thaw cycles after reconstitution.
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