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covalab/V5-Tag antibody (E10 / V4RR))/25 ug/mab90004-P

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Product Codemab90004-P
CloneE10 / V4RR
ImmunogenGKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH
Target Specificity Reacts with V5-tagged fusion proteins.
NameV5-Tag
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Product typePrimary antibodies, Tags
ClonalityMonoclonal antibody
IsotypeIgG1
Produced inMouse
SpeciesHuman, Mouse, Rat, Monkey, Chicken
LabellingNone
AppearanceLiquid
FormPurified
ConstituentsPBS 10mM, (pH 7.2)
PreservativesNaN3 0.05%
StorageShort term storage at +4°C (add 0.1% NaN3).For extended periods store in aliquots at -20°C.Antibodies are guaranteed for 6 month from date of receipt.
ApplicationsWestern Blot, Immunofluorescence, Immunocytochemistry, ELISA, Immunoprecipitation
Working dilutionsOptimal dilution should be determined by the end user. The following are guidelines only :WB (1:1000 - 1:3000)ELISA (1:100 - 1:2000)ICC/IF(1:500 - 1:2000)IP (1:10 - 1:500)
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WB - V5-Tag antibody (E10 / V4RR)<br/>(mab90004-P)<br/>Anti-V5-Tag antibody WB staining of HEK293 cells (1) untransfected or (2)transfected with V5-tagged protein vector.

  • (mab90004-P)Anti-V5-Tag antibody WB staining of HEK293 cells (1) untransfected or (2)transfected with V5-tagged protein vector.">
  • (mab90004-P)Anti-V5-Tag antibody WB staining of 15 µg of 293T cell lysates, from cells transfected with a V5-Wnt3a or V5-Vstm2 construct for the indicated number of hours, per well.">
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