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Cyanagen/IR-BLOT 800 Goat anti-Mouse/F1L108

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Properties for IR-BLOT 800 Goat anti-Mouse

Description	Highly cross adsorbed Goat anti-Mouse IgG (H&L) - IR-BLOT 800, conjugated secondary antibody
Features	Highly cross-adsorbed antibodiesHigh sensitivity and low backgroundQuantitative Western blottingOptimized for multicolor IR-Western blotting
Cross-adsorbed species	Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins
Exc-Em (nm)	774-790 nm (abs-em)
Applications	Infrared quantitative Western blotting
Content	0.1 mg of fluorescent secondary antibody lyophilized in phosphate buffer saline, pH 7.4. Contains 10 mg/mL BSA as stabilizer and 0.01% sodium azide as preservative, after reconstitution
Shipping Condition	Room Temperature
Storage	Protect from light. Store at +4 °C prior to reconstitution. Once reconstituted, stable for up to 6 months at +4 °C

Online Resources & Supporting Documents

FILTERSShow allCoAMSDSProduct ManualTechnical Note

CoA MA24A-AR F1L108 IR Blot 800 Goat Anti Mouse

CoA AR2-54 F1L108 IR Blot Goat Anti Mouse

CoA LN14A-AR FiL108 IR Blot Goat Anti Mouse

CoA ME09A-AR F1L108 IR Blot 800 Goat Anti Mouse

CoA ME30A-AR F1L108 IR Blot Goat Anti Mouse

CoA MG29A-AR F1L108 IR Blot 800 Goat Anti Mouse

MSDS F2H108 IR Blot 800 Goat Anti Mouse EN

IR Blot Product Manual

Quick Product Manual IR BLOT

IR BLOT Technical Note Rev00 20191021

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Multiplex fluorescent western blot for the detection of Vinculin and HSP-90 on HeLa whole cell lysates with Cyanagen IR-BLOT Secondary antibodies

Sample: 2-fold dilution series of HeLa whole cell lysate (abcam®) from 5μg to 0.078 μg of total protein. Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad). Blocking: 3% ECL™ Blocking Agent (GE Healthcare) in PBS. Primary antibody: Rabbit anti-Human HSP-90 (Santa Cruz Biotechnology) 1:4000 and Mouse anti-Human Vinculin (Sigma) 1:5000. Secondary antibody: IR-BLOT 700 Goat anti-Rabbit (red) and IR-BLOT 800 Goat anti- Mouse (green)1:25000. Imager: ODISSEY ® CLX – LI-COR.

Fluorescent Western blot with Cyanagen IR-BLOT and LI-COR IRDye® Secondary antibodies

Sample: 2-fold dilution series of HeLa whole cell lysate (abcam®) from 5μg to 0.078 μg of total protein. Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad). Blocking: 3% ECL™ Blocking Agent (GE Healthcare) in PBS. Primary antibody: Rabbit anti-Human HSP-90 (Santa Cruz Biotechnology) 1:4000 or Mouse anti-Human Vinculin (Sigma) 1:5000. Secondary antibody: IR-BLOT 800 Goat anti-Mouse (Cyanagen) and IRDye® 800CW Goat anti-Mouse (LI-COR) 1:25000. Imager: ODISSEY ® CLX – LI-COR.

Sample: 2-fold dilution series of HeLa whole cell lysate (abcam®) from 5μg to 0.078 μg of total protein. Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad). Blocking: 3% ECL™ Blocking Agent (GE Healthcare) in PBS. Primary antibody: Rabbit anti-Human HSP-90 (Santa Cruz Biotechnology) 1:4000 or Mouse anti-Human Vinculin (Sigma) 1:5000. Secondary antibody: IR-BLOT 800 Goat anti-Mouse (Cyanagen) and IRDye® 800CW Goat anti-Mouse (LI-COR) 1:25000. Imager: ODISSEY ® CLX – LI-COR.">

Fluorescent Western blot with Cyanagen IR-BLOT and LI-COR IRDye® Secondary antibodies. Sample: 2-fold dilution series of HeLa whole cell lysate (abcam®) from 5μg to 0.078 μg of total protein. Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad). Blocking: 3% ECL™ Blocking Agent (GE Healthcare) in PBS. Primary antibody: Rabbit anti-Human HSP-90 (Santa Cruz Biotechnology) 1:4000 or Mouse anti-Human Vinculin (Sigma) 1:5000. Secondary antibody: IR-BLOT 800 Goat anti-Mouse (Cyanagen) and IRDye® 800CW Goat anti-Mouse (LI-COR) 1:25000. Imager: ODISSEY ® CLX – LI-COR.

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Cyanagen/IR-BLOT 800 Goat anti-Mouse/F1L108

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