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Cyanagen/IR-BLOT 700 Goat anti-Mouse/F2H112

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Properties for IR-BLOT 700 Goat anti-Mouse

Description	Highly cross adsorbed Goat anti-Mouse IgG (H&L) - IR-BLOT700, conjugated secondary antibody
Features	Highly cross-adsorbed antibodiesHigh sensitivity and low backgroundQuantitative Western blottingOptimized for multicolor IR-Western blotting
Cross-adsorbed species	Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins
Exc-Em (nm)	678-690 nm (abs-em)
Applications	Infrared quantitative Western blotting
Content	0.1 mg of fluorescent secondary antibody lyophilized in phosphate buffer saline, pH 7.4. Contains 10 mg/mL BSA as stabilizer and 0.01% sodium azide as preservative, after reconstitution
Shipping Condition	Room Temperature
Storage	Protect from light. Store at +4 °C prior to reconstitution. Once reconstituted, stable for up to 6 months at +4 °C

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CoA MG23A-AR F2H112 IR Blot 700 Goat Anti Mouse

CoA LN05A-AR F2H112 IR Blot 700 Goat Anti Mouse

CoA MB28A-AR F2H112 IR Blot 700 Goat Anti Mouse

CoA ME30A-AR F2H112 IR Blot 700 Goat Anti Mouse

CoA MF06A-AR F2H112 IR Blot 700 Goat Anti Mouse

CoA MF11A-AR F2H112 IR Blot 700 Goat Anti Mouse

CoA ML07A-AR F2H112 IR Blot 700 Goat Anti Mouse

MSDS F2H112 IR Blot 700 Goat Anti Mouse EN

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Multiplex fluorescent western blot for the detection of Vinculin and HSP-90 on HeLa whole cell lysates with Cyanagen IR-BLOT Secondary antibodies.

Sample: 2-fold dilution series of HeLa whole cell lysate (abcam®) from 5μg to 0.078 μg of total protein. Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad). Blocking: 3% ECL™ Blocking Agent (GE Healthcare) in PBS.Primary antibody: Rabbit anti-Human HSP-90 (Santa Cruz Biotechnology) 1:4000 and Mouse anti-Human Vinculin (Sigma) 1:5000. Secondary antibody: IR-BLOT 700 Goat anti-Mouse (red) and IR-BLOT 800 Goat anti-Rabbit (green) 1:25000.Imager: ODISSEY ® CLX – LI-COR.

Sample: 2-fold dilution series of HeLa whole cell lysate (abcam®) from 5μg to 0.078 μg of total protein.Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad). Blocking: 3% ECL™ Blocking Agent (GE Healthcare) in PBS. Primary antibody: Rabbit anti-Human HSP-90 (Santa Cruz Biotechnology) 1:4000 and Mouse anti-Human Vinculin (Sigma) 1:5000. Secondary antibody: IR-BLOT 700 Goat anti-Mouse (red) and IR-BLOT 800 Goat anti-Rabbit (green) 1:25000. Imager: ODISSEY ® CLX – LI-COR.">

Multiplex fluorescent western blot for the detection of Vinculin and HSP-90 on HeLa whole cell lysates with Cyanagen IR-BLOT Secondary antibodies. Sample: 2-fold dilution series of HeLa whole cell lysate (abcam®) from 5μg to 0.078 μg of total protein.Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad). Blocking: 3% ECL™ Blocking Agent (GE Healthcare) in PBS. Primary antibody: Rabbit anti-Human HSP-90 (Santa Cruz Biotechnology) 1:4000 and Mouse anti-Human Vinculin (Sigma) 1:5000. Secondary antibody: IR-BLOT 700 Goat anti-Mouse (red) and IR-BLOT 800 Goat anti-Rabbit (green) 1:25000. Imager: ODISSEY ® CLX – LI-COR.

Fluorescent Western blot with Cyanagen IR-BLOT and LI-COR IRDye® Secondary antibodies

Sample: 2-fold dilution series of HeLa whole cell lysate (abcam®) from 5μg to 0.078 μg of total protein. Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad). Blocking: 3% ECL™ Blocking Agent (GE Healthcare) in PBS.Primary antibody: Rabbit anti-Human HSP-90 (Santa Cruz Biotechnology) 1:4000 or Mouse anti-Human Vinculin (Sigma) 1:5000. Secondary antibody: IR-BLOT 700 Goat anti-Mouse (Cyanagen) and IRDye® 680RD Goat anti-Mouse (LI-COR) 1:25000. Imager: ODISSEY ® CLX – LI-COR.

Sample: 2-fold dilution series of HeLa whole cell lysate (abcam®) from 5μg to 0.078 μg of total protein. Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad). Blocking: 3% ECL™ Blocking Agent (GE Healthcare) in PBS. Primary antibody: Rabbit anti-Human HSP-90 (Santa Cruz Biotechnology) 1:4000 or Mouse anti-Human Vinculin (Sigma) 1:5000. Secondary antibody: IR-BLOT 700 Goat anti-Mouse (Cyanagen) and IRDye® 680RD Goat anti-Mouse (LI-COR) 1:25000. Imager: ODISSEY ® CLX – LI-COR.">

Fluorescent Western blot with Cyanagen IR-BLOT and LI-COR IRDye® Secondary antibodies. Sample: 2-fold dilution series of HeLa whole cell lysate (abcam®) from 5μg to 0.078 μg of total protein. Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Bio-Rad). Blocking: 3% ECL™ Blocking Agent (GE Healthcare) in PBS. Primary antibody: Rabbit anti-Human HSP-90 (Santa Cruz Biotechnology) 1:4000 or Mouse anti-Human Vinculin (Sigma) 1:5000. Secondary antibody: IR-BLOT 700 Goat anti-Mouse (Cyanagen) and IRDye® 680RD Goat anti-Mouse (LI-COR) 1:25000. Imager: ODISSEY ® CLX – LI-COR.

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Cyanagen/IR-BLOT 700 Goat anti-Mouse/F2H112

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