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DescriptionMW=16.6 kDa calculated. Full length Calmodulin, cloned from human cDNA, expressed in E. coli. The protein consists of the human Calmodulin (residues 1-148, UniProtKB accession P62158).
Sequence
10 20 30 40 50 MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ 60 70 80 90100 DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY110120130140ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTA
Available mutantsN60D – CAT # G02CLM60
Purity> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 14.4 and 18.4 kDa.
Supplied as1.0 mg/mL solution in Tris 20 mM pH 8.0, NaCl 150 mM, CaCl2 2 mM, Complete Protease Inhibitor Cocktail EDTA-free (Roche). The concentration is calculated by the analysis of the absorbance at 280 nm (ε280= 2980 M-1cm-1 calculated). It is strongly recommended to add 1 tablet of Complete Protease Inhibitor Cocktail EDTA-free (Roche) in 1000 mL of buffer in case of buffer exchange.
Storage-20°C. The protein is stable at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C.Avoid repeated freeze/thaw cycles.
ReferencesBertini, I. et al. Proc. Natl. Acad. Sci. 101 (18), 6841-6846 (2004).Ehlers, M.D. & Augustine, G.J. Nature 399 (6732), 105-108 (1999).Kuboniwa, H. et al. Nat. Struct. Biol. 2 (9), 768-776 (1995).
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vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
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vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn