Overview
The E.Z.N.A.® Tissue DNA Kit offers a versatile and cost-effective method for the isolation of DNA from a wide variety of samples including fresh or frozen animal cultured cells and tissues, buccal swabs, whole blood, mouse tail snips, etc. The DNA purification process is simplified with HiBind® Mini Column technology into four quick “lyse-bind-wash-elute” steps and can be accomplished in less than 20 minutes post-lysis. This convenient spin-column format avoids time-consuming steps like alcohol precipitation, use of toxic compounds such as phenol and chloroform and allows for multiple samples to be processed in parallel. DNA purified using this kit is ready for most downstream applications such as PCR, sequencing, genotyping, southern blot analysis and restriction enzyme digestion.
- Rapid – DNA isolation in less than 20 minutes post-lysis
- Versatile – Single kit for multiple sample types
- Specialized buffer system – Optimized buffers for higher yields
- Safe – No phenol/chloroform extractions
- High-quality – DNA is suitable for a variety of downstream applications
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| Features | Specifications |
|---|---|
| Downstream Applications | PCR, sequencing, genotyping, southern blot analysis and restriction enzyme digestion |
| Starting Material | Tissues, cultured cells, mouse tail snips, paraffin-embedded tissues, whole blood, body fluids, buccal swabs |
| Starting Amount | 30 mg, or 5 x 106 cultured cells |
| Elution Volume | 100-200 μL |
| DNA Binding Technology | Silica mini spin column |
| Throughput | 1-24 |
| Processing Mode | < 20="" min=""> |
Kit Components
| Item | Available Separately |
|---|---|
| HiBind® DNA Mini Columns | View Product |
| 2 mL Collection Tubes | View Product |
| BL Buffer | View Product |
| TL Buffer | View Product |
| HBC Buffer | View Product |
| DNA Wash Buffer | View Product |
| Elution Buffer | View Product |
| Proteinase K Solution | View Product |
Protocol and Resources
Product Documentation & Literature
PROTOCOL
D3396 E.Z.N.A.® Tissue DNA Kit
SDS
D3396 SDS
QUICK GUIDE
D3396 Quick Guide
SALES SHEET
Product Data
Performance Comparison of E.Z.N.A.® Tissue DNA Kit Against Leading Competitors
Figure 1. Purified genomic DNA from 10 mg rat kidney tissue was isolated using kits from Company T, Company A, Company P, Company Q, and Omega Bio-tek’s E.Z.N.A.® Tissue DNA Kit following manufacturer’s recommended protocols. 3% of eluted DNA was analyzed on a 0.8% agarose gel. M:Lambda-Hind III.
Real-time PCR of Genomic DNA Isolation with E.Z.N.A.® Tissue DNA Kit
Figure 2. Genomic DNA was isolated from 10 mg of rat kidney with Omega Bio-tek’s E.Z.N.A.® Tissue DNA Kit. Serial dilutions of recovered genomic DNA were used as templates for real-time PCR amplification of a 100 bp fragment of the GAPDH gene with SYBR® Green labeling. Each reaction was performed in triplicate. The fluorescence versus cycle number is plotted and the 5 curves correspond to the input DNA template amounts of 10, 2, 0.4, 0.08, and 0.0016 ng.
Genomic DNA Yield From Mouse Liver
Table 1. Genomic DNA was extracted from 30 mg mouse liver using the E.Z.N.A. Tissue DNA Kit Protocol for Tissue samples and eluted in 100 µL volume. DNA concentration was determined by optical density measurements using Thermo Scientific’s NanoDrop ™ 2000c system.
Intact, high-molecular weight DNA as analyzed on an Agarose Gel
Figure 3. Genomic DNA was extracted from 30 mg mouse liver using the E.Z.N.A. Tissue DNA Kit Protocol for Tissue samples and eluted in 100 µL volume. 5 µL eluate was analyzed on a 1% Agarose gel.
Real-time PCR analysis of extracted DNA
Figure 4. Genomic DNA was extracted from 30 mg mouse liver using the E.Z.N.A. Tissue DNA Kit Protocol for Tissue samples. 2 µL of Eluted DNA was diluted 10- and 100-fold and used as a template in a 20 µL SYBR® qPCR reaction. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template DNA in free of inhibitors.
Citations
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- Guo, N., Lou, F., Ma, Y., Li, J., Yang, B., Chen, W., … & Shi, R. (2016). Circulating tumor DNA detection in lung cancer patients before and after surgery. Scientific reports, 6, 33519.
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