Overview
The Mag-Bind® Universal Pathogen 96 Kit allows rapid and reliable isolation of high-quality host genomic DNA, gram-positive and negative bacterial DNA, fungal spore DNA, viral DNA and viral RNA from tissue, urine, serum, and fecal samples.
The Mag-Bind® Universal Pathogen 96 Kit has a mechanical homogenization step using ceramics beads ranging from 0.1 mm to 2.7 mm to effectively lyse a variety of pathogens. The formulated lysis buffers do not foam during lysis allowing for better homogenization of samples.
The system allows for automation after sample lysis via Hamilton STAR™, Thermo KingFisher™ Flex, Applied Biosystems® MagMAX™, Qiagen Biosprint, and other liquid handling instruments. The system combines the Mag-Bind® technology with RBB Buffer to eliminate PCR inhibiting compounds within the samples. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. There are no organic extractions, thus reducing plastic waste and hands-on time and multiple samples can be processed in parallel.
- Ceramic Beads Pre-Aliquoted to 96-well Plates
- Isolates Yeast, Fungal, Bacterial, and Viral DNA
- Isolate Viral RNA
- Hamilton Microlab® STAR
- Hamilton Microlab® NIMBUS
- KingFisher™, BioSprint®, and MagMAX® 96
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| Features | Specifications |
|---|---|
| Starting Material | Plasma/Serum,Tissue, Fecal, Urine |
| Starting Amount | 250 µL plasma/serum/stool, 30 mg tissue |
| Elution Volume | 50-100 μL |
| Technology | Magnetic Beads |
| Processing Mode | Automated, Manual |
| Throughput | 96 |
Kit Components
| Item | Available Separately |
|---|---|
| E-Z 96 Disruptor Plate C Plus | View Product |
| Caps for Racked Microtubes | --- |
| Mag-Bind® Particles RQ | Call for Pricing |
| SLX-Mlus Buffer | View Product |
| DS Buffer | Call for Pricing |
| PCP Buffer | --- |
| XP2 Buffer | View Product |
| RBB Buffer | View Product |
| VHB Buffer | View Product |
| SPM Wash Buffer | View Product |
| Elution Buffer | View Product |
| Proteinase K Solution | View Product |
| RNase A | View Product |
Protocol and Resources
Product Documentation & Literature
PROTOCOL
M4029 Mag-Bind® Universal Pathogen 96 Kit
SDS
M4029 SDS
SALES SHEET
Product Data
Detection of Fungal Spores
Figure 1. Cryptosporidium oocysts were added to corresponding sample types and isolated with Mag-Bind® Universal Pathogen Kit. 20 µL SYBR® qPCR was performed in triplicate on primers specific for the target organism. Average of triplicate data shown.
Detection of Gram-Positive Bacteria
Figure 2. Group B strep cultured samples were added to corresponding sample types and isolated with Mag-Bind® Universal Pathogen Kit. 20 µL SYBR® qPCR was performed in triplicate on primers specific to the target organism. Average of triplicate data shown.
Detection of Gram-Negative Bacteria
Figure 3. E. coli cells were cultured overnight in LB broth and added to corresponding sample types and isolated with Mag-Bind® Universal Pathogen Kit. 20 µL SYBR® qPCR was performed in triplicate on primers specific to the target organism. Average of triplicate data shown.
Detection of Viral DNA
Figure 4. HBV viruses were added to corresponding sample types and isolated with Mag-Bind® Universal Pathogen Kit. 20 µL SYBR® qPCR was performed in triplicate on primers specific to the target organism. Average of triplicate data shown.
Detection of Viral RNA
Figure 5. Influenza A/B virus was added to corresponding sample types and isolated with Mag-Bind® Universal Pathogen Kit. 20 µL SYBR® qPCR was performed in triplicate on primers specific to the target organism. Average of triplicate data shown.