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Viafluor™ SECellProliferationKitsuseamine-reactivedyestocovalentlylabelthecellcytoplasm,andcanbeusedtomonitorcelldivisionbyflowcytometry.
Themembranepermeablecompoundisnon-fluorescentuntilitentersviablecells,whereitishydrolyzedbycytoplasmicesteraseenzymestoreleasesthefluorescentamine-reactivedye.Thedyesthencovalentlyreactwithaminegroupsonintracellularproteins,formingfluorescentconjugatesthatareretainedinthecell,whileexcessunreacteddyeiswashedaway. Immediatelyafterstaining,asingle,brightfluorescentpopulationwillbedetectedbyflowcytometry.Witheachcelldivision,daughtercellsinheritroughlyhalfofthefluorescentlabel,allowingthenumberofcelldivisionsthatoccurafterlabelingtobedetectedbytheappearanceofsuccessivelydimmerfluorescentpeaksonaflowcytometryhistogramcomparedtocellsanalyzedimmediatelyafterstaining(Figs.1and2). Cellproliferationdyescanbeusedtotrackcelldivisionsinvivoorinvitro.ThestainingalsocanwithstandfixationandpermeABIlizationforsubsequentimmunostaining.
KitComponents:
Eachkitcontains10lyophilizeddyevials,anhydrousDMSOforpreparingstocksolutions,andalabelingprotocol.Onekitcanbeusedtolabelatleast1.8 108cells;thefinalnumberofassaysthatcanbeperformedperkitdependsonthedyeconcentrationused(seeproductprotocolunderdownloads).
ViaFluorkitscomein3dyeoptions:
ViaFluor™CFSE(alsoknownasCFDA-SE)canbedetectedintheFITCchannel.ViaFluor™405-SEcanbedetectedinthePacificBluechannel.ViaFluor™568-SEcanbedetectedintheTexasRedorPE-TexasRedchannels.ViaFluor™CFSEcanbeusedasacytoplasmicstaininyeast.HoweverViaFluor™405-SEandViaFluor™568-SEdonotstainwellinyeast.
Carboxyfluoresceindiacetate,succinimidylester(CFDA-SEorCFSE)isalsoavailableseparately.
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Innovation
At Biotium, innovation is at the very heart of what we do every day. As a small technology company, we understand the importance of innovation to our competitiveness and growth. The company devotes a significant amount of resources to research and development and intellectual property protection. Our efforts have resulted in a growing number of granted and pending patents that cover a number of industry-leading technologies, including the following:
GelRed™ and GelGreen™ nucleic acid gel stains: highly sensitive DNA/RNA gel stains made safe by preventing dye penetration into cells
EvaGreen™ DNA-binding Dye: qPCR dye working on a novel “release-on-demand” DNA-binding mechanism; the only dye that works perfectly for both qPCR and highly sensitive melt curve analysis
CF™ dyes: A series of 20 highly water soluble dyes with colors spanning the entire visible and near infrared spectra; designed for protein labeling using breakthrough chemistries to produce unrivaled performance in brightness and photostability
Mix-n-Stain™ Antibody Labeling Kits: A revolutionary protein labeling technology that allows anyone to optimally label an antibody , with or without the presence of BSA or gelatin, with one of our CF™ dyes or biotin in 30 minutes and ~100% yield.
NucView™ Caspase 3/7 Enzyme Substrate: A novel concept to detect caspase activity in live cells by releasing a cell nucleus-staining dye upon substrate cleavage
AccuClear™ and AccuBlue™ DNA Quantitation Kits: Kits with the lowest dsDNA detection limit and widest linear range in the market
PMA™ DNA Modification Agent: An essential reagent for selective detection of viable pathogens by qPCR
Product Quality and Services
Biotium has state-of-the-art facilities and, more importantly, a team of highly experienced scientists and supporting staff to provide high quality products and superior service. Our chemistry and analytical labs employ advanced purification and analytical instruments to ensure product purity and consistency. Similarly, our bioscience lab is fully equipped for conducting cell imaging and bioanalytical experiments, such as confocal microscopy, gel electrophoresis, flow cytometry, real-time PCR, and fluorescent/bioluminescent plate-based assays. These capabilities enable us to produce user-friendly product protocols and provide superior technical support to our customers.
Business Partnering
Biotium has licensed several of its patented technologies to a number of major international biotech companies including Sigma-Aldrich, Agilent, BioRad, Qiagen, BD Biosciences, EMD Millipore, Fluidigm, Essen BioScience, Caliper Life Sciences, Illumina, and others. Biotium’s products are sold world-wide through our international distributors, including VWR USA and VWR Europe. In addition, we frequently collaborate with academic scientists to develop new tools for the ever-changing needs in research. These partnerships extend Biotium’s reach to more customers and expand applications of Biotium’s cutting-edge technologies in life science research, medical diagnostics and pharmaceutical science.