5-Aminoallyl-dUTP(AA-dUTP)canbeenzymaticallyincorporatedintoDNA.Theresultingamine-containingDNAcanbesubsequentlylabeledwithafluorescentdye,biotinorotherhaptensviaconventionalpeptidecouplingmethod(1,2).Thistwo-stepmethodforlabelingnucleicacidsisconsiderablymoreeconomicalthantheone-stepmethodusingaprelabeleddUTP.
AA-dUTPisalsoavailableinlyophilizedform(catalogno.40020-1).
1.BiolChemHoppeSeyler371,953(1990).
2.Biotechniques28,518(2000).
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn