RedDot™2 FarRed Nuclear Stain | Biotium

From:106Sizes:5-20uglabeling,10-20uglabeling,20-50uglabeling,25-50uglabeling,50-100uglabelingCatalog#:92433,92432,92431,92430,92429,92428,92427,92426,92425,92230,92231,92232,92233,92234,92235,92236,92237,92238,92239,92240,92241,92242,92243,92245,92246,92247,92248,92250,92251,92252,92253,92254,92255,92256,92257,92258,92259,92260,92261,92262,92263,92264,92265,92267,92268,92270,92271,92272,92273,92274,92275,92276,92277,92278,92279,92280,92281,92282,92283,92284,92285,92287,92288,92289,92290,92291,92303,92304,92305,92316,92317,92318,92319,92320,92321,92322,92323,92324,92331,92332,92333,92334,92335,92336,92337,92338,-92339ViewallHide
From:120Sizes:5-20uglabeling,20-50uglabelingCatalog#:92501,92500,92502,92503,92504,92505,92506,92507,92508,92509,92510,92511,92512,92513,92514,-92515ViewallHide
Afar-redcellmembrane-impermeantnucleardyesimilartoDraq7.IdealforstainingthenucleiofdeadorfixedandpermeabilizedcellswithminimalcytoplasmicRNAstaining.
Fixationoptions

Permeabilizebeforestaining,Fixbeforestaining(methanol),Fixbeforestaining(formaldehyde)

WherecanIfindtheexpirationdateorshelflifeofaproduct?

Biosciencekits
Theguaranteedshelflifefromdateofreceiptforbiosciencekitsislistedontheproductinformationsheet.Somekitshaveanexpirationdateprintedonthekitboxlabel,thisistheguaranteedshelflifedatecalculatedfromthedaythattheproductshippedfromourfacility.Kitsoftenarefunctionalforsignificantlylongerthantheguaranteedshelflife.Ifyouhaveanolderkitinstoragethatyouwishtouse,werecommendperformingasmallscalepositivecontrolexperimenttoconfirmthatthekitstillworksforyourapplicationbeforeprocessingalargenumberofsamplesorprecioussamples.

Antibodiesandotherconjugates
Theguaranteedshelflifefromdateofreceiptforantibodiesandconjugatesislistedontheproductinformationsheet.Antibodiesandotherconjugatesoftenarefunctionalforsignificantlylongerthantheguaranteedshelflife.Ifyouhaveanolderconjugateinstoragethatyouwishtouse,werecommendperformingasmallscalepositivecontrolexperimenttoconfirmthattheproductstillworksforyourapplicationbeforeprocessingalargenumberofsamplesorprecioussamples.

Forlyophilizedantibodies,werecommendreconstitutingtheantibodywithglycerolandantimicrobialpreservativelikesodiumazideforthelongestshelflife(notethatsodiumazideisnotcompatiblewithHRP-conjugates).

Chemicals,dyes,andgelstains
Biotiumguaranteesthestabilityofchemicals,dyes,andgelstainsforatleastayearfromthedateyoureceivetheproduct.However,themajorityoftheseproductsarehighlystableformanyyears,aslongastheyarestoredasrecommended.Storageconditionscanbefoundontheproductinformationsheetorproductsafetyanddatasheet,materialsafetydatasheet,andontheproductlabel.Fluorescentcompoundsshouldbeprotectedfromlightforlongtermstorage.

IfyouhaveaBiotiumcompoundthathasbeeninstorageforlongerthanoneyearthatyouwishtouse,werecommendperformingasmallscalepositivecontrolexperimenttoconfirmthatthecompoundstillworksforyourapplicationbeforeprocessingalargenumberofsamplesorprecioussamples.

Expirationdatebasedondateofmanufacture(DOM)
Ifyourinstitutionrequiresyoutodocumentexpirationdatebasedondateofmanufactureforreagents,pleasecontacttechsupport@biotium.comforassistance.

Chemicalproductswithspecialstabilityconsiderations:

Esters

Estercompoundsincludethefollowing:
•Succinimidylesters(SE,alsoknownasNHSesters),suchasouramine-reactivedyes
•Acetoxymethylesters(AMesters)suchasourmembrane-permeableionindicatordyes
•Diacetate-modifieddyes,likeViaFluor™405,CFDA,andCFDA-SEcellviability/cellproliferationdyes

Esterdyesarestableinsolidformaslongastheyareprotectedfromlightandmoisture.Estersarenotstableinaqueoussolution.ConcentratedstocksolutionsshouldbepreparedinanhydrousDMSO(seeBiotiumcatalogno.90082).StocksolutionsinanhydrousDMSOcanbestoreddesiccatedat-20°Cforonemonthorlonger.Estersshouldbedilutedinaqueoussolutionimmediatelybeforeuse.Succinimidylesters(SE)shouldbedissolvedinasolutionthatisfreeofamine-containingcompoundslikeTris,glycine,orprotein,whichwillreactwiththeSEfunctionalgroup.AMestersanddiacetatecompoundsshouldbedissolvedinasolutionthatisfreeofserum,becauseserumcouldcontainesterasesthatwouldhydrolyzethecompound.

AnoteonCF™dyesuccinimidylesterstability
Succinimidylestersaregenerallysusceptibletohydrolysis,whichcanresultinlowerlabelingefficiency.Heavilysulfonateddyes,suchastheAlexaFluor®dyes,DyLight®dyesandIRDyes®areparticularlyhygroscopic,worseningthehydrolysisproblem.Forexample,thepercentofactiveAlexaFluor®488succinimidylester(SE)couldbewellbelow50%bythetimeofapplication(accordingtothemanufacturer’sproductdatasheet).InanumberofAlexaFluor®SEreactivedyes,theSEgroupisderivedfromanaromaticcarboxylicacid,whileinallofBiotium’sCF™dyestheSEgroupispreparedfromanaliphaticcarboxylicacid.ThisstructuraldifferencereducesthesusceptibilityofCF™dyeSEreactivegroupstohydrolysis,resultinginrelativelystablereactivedyeswithconsistentlyhigherlabelingefficiencycomparedtootherSEderivativesofotherfluorescentdyes.

Maleimides,MTSandthiosulfatedyes
Likethesuccinimidylesterdyes,thesedyesarealsosusceptibletohydrolysis,althoughgenerallytoamuchlowerdegree.Thus,forlongtermstorage,anhydrousDMSOisrecommendedformakingstocksolutions.

Otherreactivedyes
Amines,aminooxy(alsoknownasoxylamine),hydrazide,azide,alkyne,BCN,andtyramidereactivedyes,aswellasdyefreeacids,aregenerallystableinaqueoussolutionwhenstoredat-20°Cfor6-12monthsorlonger,aslongasnocompoundsarepresentthatmayreactwiththedye’sfunctionalgroup.Seetheproductinformationsheetsforspecificreactivedyesmoreinformation.

CoelenterazinesandD-luciferin

Coelenterazinesarestableinsolidformwhenstoredasrecommended;theyarenotstableinaqueoussolution.Concentratedcoelenterazinestocksolutions(typically1-100mg/mL)shouldbepreparedinethanolormethanol;donotuseDMSOorDMFtodissolvecoelenterazines,becausethesesolventswilloxidizethecompounds.Ethanolormethanolstocksofcoelenterazinecanbestoredat-20°Corbelowforsixmonthsorlonger;alcoholstocksmayevaporateduringstorage,sousetightlysealingscrewcapvialsandwrapthevialswithParafilmforlongtermstorage.Propyleneglycolalsocanbeusedasasolventtominimizeevaporation.Ifthesolventevaporates,thecoelenterazinewillstillbepresentinthevial,sonotethevolumeinthevialpriortostoragesothatyoucanadjustthesolventvolumetocorrectforevaporationifneeded.Prepareworkingsolutionsinaqueousbuffersimmediatelybeforeuse.Coelenterazinesarestableforuptofivehoursinaqueoussolution.

Aquaphile™coelenterazinesarewatersolubleformulationsofcoelenterazines.Theyarestableinsolidformwhenstoredasrecommended.Aquaphile™coelenterazinesshouldbedissolvedinaqueoussolutionimmediatelybeforeuse.Theyarestableforuptofivehoursinaqueoussolution.

Notethatcoelenterazinesarepredominantlyyellowsolids,butmaycontaindarkredorbrownflecks.Thisdoesnotaffectproductstabilityorperformance.Ifyourcoelenterazineisuniformlybrown,thenitisoxidizedandneedstobereplaced.

D-luciferinisstableinsolidformandasaconcentratedstocksolutionwhenstoredasrecommended;itisnotstableatdiluteworkingconcentrationsinaqueoussolution.PrepareconcentratedD-luciferinstocksolutions(typically1-100mg/mL)inwater,andstoreinaliquotsat-20°Corbelowforsixmonthsorlonger.Prepareworkingsolutionsimmediatelybeforeuse.

Category:CF®Dyes,Mix-n-Stain™AntibodyLabelingKits,NucView®Caspase-3EnzymeSubstrates,DNAQuantitationKits,LuciferaseAssays,Productshipping,storage,shelflife,solubility
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Theproductlabelsaysprotectfromlight,whyisntitinanambervial?

Someofourlargevolumelight-sensitiveproductsarepackagedinamberbottlesoramberglassvials,whichareeasytohandleandprotecttheproductsfromlight.However,ambermicropackagingvials(0.5mLor2mL)makeitverydifficulttoseesmallquantitiesofdyewhenpreparingsolutionsorpipetting.Therefore,weonlypackagephotoreactivedyeslikePMAorPMAxxinambermicropackagingvials.Someofourreactivedyesarepackagedintransparentvials,thensealedinsideamoisture-resistantfoilbag,whichalsowillprotectthemfromlight.

Otherfluorescentdyesintransparentvialsshouldstoredinthedarkforlongtermstorage.Itsfinetostoredyevialsuncoveredinawindowlessrefrigeratororfreezerwithanautomaticlightshut-off.Ifdyeswillbestoredinaglass-front(deli-style)refrigerator,walk-inrefrigerator/freezer,oratroomtemperaturewithconstantlightexposure,theyshouldstoredinanon-transparentbox(likeawhitecardboardfreezerbox),inacloseddrawer,inablackplasticbag,orcoveredwithaluminumfoil.

Fluorescentdyesgenerallyarenotsensitivetobrieflightexposurewhiletheyarebeinghandledonthebenchduringanexperiment.Tobeonthesafeside,weusuallylooselycovertuberackswithapieceoffoilifthedyevialsaregoingtobeoutonthebenchformorethan30minutesorso. Butmostdyesarestableenoughthatevenaccidentallyleavingthemonthebenchforadaywillnotaffectperformance.WhenhandlingphotoreactivedyeslikeEMA,PMA,orPMAxx,wetaketheextraprecautionofdimmingthelights.

Category:Productshipping,storage,shelflife,solubility
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Shipping,Handling&Tax(CAonly)notincludedinprice.Learnmore

TaxisassessedinthestateofCalifornia.Allfeeswillbecalculatedduringcheckoutbasedonyourshippinginformationandaresubjecttochange.

RedDot2isafar-redcellmembrane-impermeantnucleardyesimilartoDraq7.IdealforstainingthenucleiofdeadorfixedandpermeabilizedcellswithminimalcytoplasmicRNAstaining.

Cellmembrane-impermeantIdealforspecificallystainingthenucleiofdeadcellsMinimalcytoplasmicRNAstainingλEx/λEm(DNA-bound)=665/695nm,fordetectionintheCy®5channel

RedDot2providesexcellentnuclearcounterstaininginfixedandpermeabilizedcellsandtissuesectionswithouttheneedforRNasetreatmenttoremovecytoplasmicRNA.Thedyeishighlythermostableandphotostable,providingconvenienthandlingandidealfordemandingapplicationssuchasconfocalmicroscopy.RedDot2canbeefficientlyexcitedbywavelengthsfrom488to647nm,andthereforecanbeusedwiththe488nmflowcytometrylaserline.

RedDot2 isdeadcellspecificinallcelltypes,includingmammaliancells,bacteriaandyeast.SeeourCellularStainsTable formoreinformationonhowourdyesstainvariousorganisms.

Pleasenote:far-reddyeslikeRedDot2arenotvisibletothehumaneye,butmustbeimagedwithaCCDcameraorbyconfocalmicroscopy.

PleasealsoseeRedDot1(catalogno.40060),aspectrallysimilardyedesignedforspecificnuclearstainingoflivecells.

RedDot1andRedDot2Far-RedNuclearStains

InCellWesternisatrademarkofLI-COR®Biosciences.CydyeisaregisteredtrademarkofGEHealthcare.Draq7isatrademarkofBioStatusLtd.

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DAPIisapopularbluefluorescentDNAdye.ThedyebindstotheminorgrooveofdsDNAwithapproximately20-foldfluorescenceenhancement.DetailedView
ArevolutionaryantifademountingmediumoptimallyformulatedforpreservingfluorescenceofourCF®dyesandotherfluorochromes.AvailablewithorwithoutDAPI.DetailedView
NucSpot®LiveCellNuclearStainsarecell-membranepermeableDNAdyesthatspecificallystainsnucleiinliveorfixedcells.TheyhaveexcellentspecificityforDNAwithouttheneedforawashstep,andtheyhavelowtoxicityforlivecellimaging.DetailedView
Propidiumiodide(PI)isamembrane-impermeantnucleicacidintercalator.Thedyeiscommonlyusedtoselectivelystaindeadcellsinamixedlive-deadpopulationandalsousedasacounterstaininmulticolorfluorescentassays.DetailedView
Afar-redcellmembrane-permeablenucleardyesimilartoDraq5thatisidealforspecificallystainingthenucleioflivecells.DetailedView
BiotiumimplementsaQualitySystem,certifiedbyQASaccordingtoStandardQASISO9001:2015.CertificateNo.US3564