31000 EvaGreen Dye, 20X in waterBiotium|biotium荧光试剂|GelRed|CF染料|biotiumBiotium 中国区总代理上海开放生物科技有限公司

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SafetyReport(PDF):EvaGreenSafetyReport

产品介绍：

EvaGreen®dyeisagreenfluorescentnucleicaciddyewithfeaturesthatmakethedyeusefulforseveralapplicationsincludingqPCRandDNAmeltcurveanalysis,real-timemonitoringofthermophilichelicase-dependentamplification(tHDA),andcapillarygelelectrophoresis.TheDNA-bounddyehasexcitationandemissionspectraveryclosetothoseoffluorescein(FAM)orSYBR®GreenI,makingthedyereadilycompatiblewithinstrumentsequippedwiththe488nmargonlaseroranyvisiblelightexcitationwithwavelengthintheregion.EvaGreen®dyeisextremelystableboththermallyandhydrolytically,providingconvenienceduringroutinehandling.Thedyeisessentiallynonfluorescentbyitself,butbecomeshighlyfluorescentuponbindingtodsDNA.EvaGreen®dyeisnonmutagenicandnoncytotoxicbybeingcompletelyimpermeabletocellmembranes,unlikeSYBR®GreenI,whichenterscellrapidlyandisknowntobeapowerfulmutation-enhancer(Ohta,etel.Mutat.Res.492,91(2001)).

TheuniquepropertiesofEvaGreen®dyehavemadeitparticularlyusefulinquantitativereal-timePCR(qPCR)applications.ComparedwiththewidelyusedSYBR®GreenI,EvaGreen®dyeisgenerallylessinhibitorytowardPCRandlesslikelytocausenonspecificamplification.Asaresult,EvaGreen®dyecanbeusedatamuchhigherdyeconcentrationthanSYBR®GreenI,resultinginahighresolutionsignalfordropletdigitalPCR,real-timePCRandmeltcurveanalysis.

Alsoseeourready-to-useFastEvaGreen®qPCRMasterMixandFastPlusEvaGreen®qPCRMasterMixes.

EvaGreendyeandapplicationsarecoveredunderUSpatentnos.7,803,943and7,776,567andpendinginternationalpatents.

技术亮点:

LowPCRinhibition

ExhibitsmuchlessPCRinhibitionthanSYBR®GreenIviaasmart“release-on-demand”DNA-bindingtechnology

HighlySensitive

LowPCRinhibitionofthedyepermitsahigherdyeconcentrationtobeusedformuchgreaterqPCRandmeltcurveanalysissignals

Nonmutagenicandnoncytotoxic

NonmutagenicandnoncytotoxicbystandardAmestest;completelyimpermeabletocellmembranes

CompatiblewithfastPCRprotocols

MinimalinterferencetoPCRmakesitpossibletosignificantlyshortenthechainextensiontime

CompatiblewithmultiplexPCR

Nodyemigrationfromamplicontoampliconwhenusedattherecommendedconcentration

Unsurpassedthermalstability,chemicalstabilityandphotostability

NodetectabledyedecompositioninPCRbufferat95-100°Cfor48hours;highlystableundereitheralkalineoracidiccondition;withstandsrepeatedfreeze-thawcycles

SpectrallysimilartoSYBR®GreenI

CompatiblewithallmajorbrandqPCRinstrumentsandenzymesystems

点击次数：更新时间：2015-11-0315:27:03【打印此页】【关闭】上一条：40013PMAdye下一条：30066Apoptotic,NecroticandHealthyCellsQuantitationKitPlus

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Biotium中国区总代理-上海开放生物科技有限公司，专利产品和技术：CF染料新一代荧光标记染料，EvaGreen高分辨率溶解曲线饱和染料，NucView双功能荧光凋亡检测Caspase3/7，Cheetah热启动酶用于多重PCR技术和SNP检测、突变扫描等，AllGlo探针合成染料（替代Taqman技术），细胞凋亡、活性检测试剂盒。DNA定量试剂盒、蛋白定量试剂盒、核酸安全无毒灵敏染料GelRed、蛋白快速染色、神经、离子指示探针等等。