Foreaseofuseandsaferhandling,weofferbothHoechst33258andHoechst33342dissolvedinH2Oat10mg/mL.Hoechstdyesarecellmembrane-permeant,minorgroovebindingbluefluorescentDNAstains.Hoechst33258andHoechst33342arespectrallysimilar.Hoechst33528isslightlymorewatersolublethanHoechst33342,butbothdyesarewidelyusedincellcycleandapoptosisstudiesandalsoasnuclearcounterstains.Theyaretypicallyusedtostainliveorfixedcellsinbufferormediumat1ug/mL,withnowashsteprequired.
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