From: 106Sizes: 5-20 ug labeling, 10-20 ug labeling, 20-50 ug labeling, 25-50 ug labeling, 50-100 ug labelingCatalog #: 92433, 92432, 92431, 92430, 92429, 92428, 92427, 92426, 92425, 92230, 92231, 92232, 92233, 92234, 92235, 92236, 92237, 92238, 92239, 92240, 92241, 92242, 92243, 92245, 92246, 92247, 92248, 92250, 92251, 92252, 92253, 92254, 92255, 92256, 92257, 92258, 92259, 92260, 92261, 92262, 92263, 92264, 92265, 92267, 92268, 92270, 92271, 92272, 92273, 92274, 92275, 92276, 92277, 92278, 92279, 92280, 92281, 92282, 92283, 92284, 92285, 92287, 92288, 92289, 92290, 92291, 92303, 92304, 92305, 92316, 92317, 92318, 92319, 92320, 92321, 92322, 92323, 92324, 92331, 92332, 92333, 92334, 92335, 92336, 92337, 92338, - 92339View allHide
From: 120Sizes: 5-20 ug labeling, 20-50 ug labelingCatalog #: 92501, 92500, 92502, 92503, 92504, 92505, 92506, 92507, 92508, 92509, 92510, 92511, 92512, 92513, 92514, - 92515View allHide
Features
Brighter and more photostable than AMCA
Direct replacement for Alexa Fluor® 350
Highly water soluble and pH-insensitive
Features
More photostable than Pacific Blue®, with less spill-over in the green channel
An excellent choice for super-resolution imaging by SIM
Features
Photostable dyes suitable for microscopy
Perfect match for the CFP filter set
Suitable for flow cytometry in the AmCyan channel
Highly water soluble and pH-insensitive
Features
Minimally charged, for less non-specific binding than Alexa Fluor® 488
Narrower emission spectrum for less bleed to red
Very photostable
Compatible with super-resolution imaging by TIRF
Highly water soluble and pH-insensitive
Features
Image using the same settings as FITC or CF®488A
Can be distinguished from CF®488A in the same specimen by spectral imaging and linear unmixing
Excitation laser line: 532 nm
Direct replacement for: Alexa Fluor® 532, Atto 532
Features
Optimized for the 543 nm laser
Yields the brightest conjugates among spectrally similar dyes
Highly water-soluble and pH-insensitive
Excitation laser line: 532 nm or 568 nm
Direct replacement for: Alexa Fluor® 568,
ATTO 565, Rhodamine Red
Extremely photostable
Excellent choice for multi-color imaging with CF®488A and CF®640R
Compatible with TIRF and multicolor STORM super resolution imaging
Excitation laser line: 561 to 568 nm
Replaces: Alexa Fluor® 568, ATTO 565, DY-560, Rhodamine RedCF®583 Technical Summary
Abs/Em maxima: 583/606 nm
Extinction coefficient: 150,000
Molecular weight: ~3127
Excitation laser line: 561 to 568 nm
Replaces: Cy®3.5
Features
Yields the brightest antibody conjugates among spectrally
similar dyes.
Excellent choice for multicolor imaging with green dyes like CF®488A
Extremely photostable
Also see CF®594ST, a version of CF®594 engineered specifically for STORM microcopy
Highly fluorescent and extremely photostable
Absorption/emission at 617/639 nm for use in FRET or multi-color detection
Excitation laser line: 633 nm or 635 nm
Replaces: Alexa Fluor® 633, Alexa Fluor®
647, Cy®5, DyLight® 633, DyLight® 649
Features
Yields the brightest antibody conjugates among spectrally similar dyes
Far more photostable than Alexa Fluor® 647
Compatible with TIRF, FIONA, and gSHRImP super-resolution imaging methods
Features
Best photostability among Cy®5-like dyes
Yields highly fluorescent protein conjugates
Compatible with TIRF and FLIMP super-resolution microscopy
Highly water soluble and pH insensitive
Validated in multi-color super-resolution imaging by STORM
Features
CF®660C Features
Much brighter and more photostable than Alexa Fluor® 660
Compatible with multicolor super-resolution imaging by STORM
CF®660R Features
Brighter than Alexa Fluor® 660
Unrivaled photostability among spectrally similar dyes
Excitation laser line: 680 nm or 685 nm
Replaces: Alexa Fluor® 680, Cy®5.5, IR®Dye 680
CF®680R
Abs/Em Maxima: 680/701 nm
Extinction coefficient: 140,000
Molecular weight: ~ 912
Excitation laser line: 680 nm or 685 nm
Replaces: Alexa Fluor® 680, Cy®5.5, IR®Dye 680
The brightest among spectrally similar dyes
Validated in multicolor STORM and 3D super-resolution
microscopy
Compatible with LI-COR® Odyssey®
CF®680R Features
Unrivaled photostability among spectrally similar dyes
Compatible with STED and single molecule spectroscopy super-resolution imaging (see pp. 20-21)
Molecular weight compatible with nucleic acid labeling
Compatible with LI-COR® Odyssey®
Excitation laser line: 633, 635, 680 or 685 nm
Replaces: Alexa Fluor® 700, DyLight® 700
CF®750
Abs/Em Maxima: 755/777 nm
Extinction coefficient: 250,000
Molecular weight: ~ 3009
Excitation laser line: 633, 635, 680 or 685 nm
Replaces: Alexa Fluor® 750, Cy®7, DyLight® 750
CF®770
Abs/Em Maxima: 770/797 nm
Extinction coefficient: 220,000
Molecular weight: ~ 3138
Excitation laser line: 785 nm
Replaces: DyLight™ 800, IRDye 800CW
CF®790
Abs/Em Maxima: 784/806 nm
Extinction coefficient: 210,000
Molecular weight: ~ 3267
Excitation laser line: 785 nm
Replaces: Alexa Fluor® 790
CF®800
Abs/Em Maxima: 797/816 nm
Extinction coefficient: 210,000
Molecular weight: ~3334
Excitation laser line: 785 nm
Spectrally similar to: Indocyanine Green
CF®820
Abs/EmMaxima: 822/835 nm
Extinction coefficient: 253,000
Molecular weight: ~2553
Excitation laser line: 785 nm
Superior signal-to-noise for bioconjugates
Dye options for STORM photoacoustic imaging
Features
CF Dyes validated in SIM, STORM, STED, TIRF, and more
Superior brightness, photostability, and photochemical switching
Single label antibody conjugates for STORM
Growing list of publications
Growing collection of more than 1000 monoclonal antibodies. Many antibodies verified in HuProt™ Array and validated in IHC and other applications. Available with bright and photostable CF® dyes, R-PE, APC, PerCP, HRP, AP, biotin, or purified. Affordable 100 uL trial sizes.
Many host/target species and cross-absorption options, featuring a wide spectrum of our bright & photostable CF® dyes or biotin. View Category
Secondary antibody conjugates of our STORM-compatible CF® dyes, with an average degree of labeling (DOL) of one, specifically designed for super-resolution imaging by STORM. View Category
Mix-n-Stain™ kits use revolutionary technology that dramatically simplifies antibody labeling and requires no purification. The labeling is covalent and stable. Available with your choice of 24 CF® dyes, biotin, FITC, fluorescent proteins, enzymes, or haptens. View Category
Everything you need to label and purify antibodies or other proteins. Choose from 19 bright & photostable CF® dyes or biotin. View Category
Our bright, photostable, highly water soluble CF® dyes with a wide selection of colors and functional groups. View Category
Additional conjugates or reactive dye forms may be added on request, contact techsupport@biotium.com to inquire.
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What are the chemical structures of CF® dyes? The exact chemical structures of CF® dyes are currently confidential but will be fully disclosed at a later stage when pending patents become granted. In general terms, the structure of a CF® dye may be divided into two parts: a) dye core structure (i.e. the aromatic ring skeleton that defines the dye’s color or absorption/emission wavelengths), and b) core structure-modifying elements. At present, CF® dyes bear the core structures of coumarin, pyrene, rhodamine or cyanine dyes. Blue fluorescent CF® dyes are based on coumarin or pyrene dye core structure, while green to near-IR CF® dyes are based on either cyanine or rhodamine dye core structures. Core structure-modifying elements refer to various chemical attachments to the core structure and are a key aspect of the CF® dye invention that makes CF® dyes superior to other commercial dyes.
Category: CF® Dyes
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For several CF® dye colors, there is an R form and a C form, both having similar absorption and emission spectra. In such a case, which of the two CF® dyes should I choose? Rhodamine-based CF® dyes (designated R) generally have better photostability but weaker fluorescence than their cyanine-based equivalents (designated C). Therefore, rhodamine-based near-IR CF® dyes are a better choice for microscopy, while cyanine-based CF® dyes are more ideal for flow cytometry, Western blotting, and other applications where photobleaching is less of a concern. Another factor to consider is the size of the dyes. Some of the cyanine-based near-IR CF® dyes are much larger than the rhodamine-based equivalents. For antibody labeling, either version of the CF® dyes is suitable. However, for applications where the dye size may cause a steric problem, the smaller dye may be a better choice.
Category: CF® Dyes
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What is the difference between CF®405S, CF®405M, and CF®405L? All three of these dyes can be excited by the 405 nm laser (or UV mercury lamp). They differ in their emission wavelengths. CF®405S has blue fluorescence emission at 431 nm, similar to AlexaFluor® 405, Cascade Blue®, and DyLight 405 (Thermo Scientific). CF®405M has longer wavelength blue fluorescence emission at 452 nm, similar to Horizon V450 (BD Biosciences) and Pacific Blue® (Thermo Scientific). CF®405L has orange fluorescence emission at 545 nm, similar to Pacific Orange® (Thermo Scientific). We recommend choosing the dye that best fits your instrument s detection settings. See our CF® Dye Quick Reference Table and CF® Dye Selection Guide for more information on CF® dyes.
Category: CF® Dyes
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