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The antibody produces amaximum biotin-streptavidin/HRP staining at a 1/500 - 1/1000 dilution in rat anterior/intermediate pituitary. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The recommened dilution series is 1/500-1/1000 in PBS/0.3% Triton X-100 - Bn-SA/HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg/mL of ACTH.
Photo Description: IHC image of neurons staining for ACTH in the rat anterior pituitary gland. The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:1000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Host: Rabbit
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Availability: In Stock
Reacts With: Dove, Eel, Goldfish, Hamster, Human, Monkey, Mouse, Ram (Sheep), Rat, Trout
Alternate Names: anti-ACTHRRID: AB_572216Immunogen: Adrenocorticotropic HormoneGene Symbol: POMCDatabase Links:Entrez Gene: 102092785DoveEntrez Gene: 100756311 HamsterEntrez Gene: 5443 HumanEntrez Gene: 694430 MonkeyEntrez Gene: 18976 MouseEntrez Gene: 443212 Ram (Sheep)Entrez Gene: 24664 RatEntrez Gene: 100136771 TroutNCBI Gene Aliases: ACTHR,MGC12579
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