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ImmunoVision/MPO-3000/200 micrograms/MPO-3200

Product Name:   Myeloperoxidase (MPO) Antigen

Description:

The myeloperoxidase (MPO) antigen is a heme-containing protein found in the primary granules of neutrophils and monocytes. It’s primary function is to destroy phagocytosed microorganisms by generating highly reactive oxidative species within the phagosome (1). MPO is a heterodimer consisting of two 59 kD heavy chains and two 14 kD light chains. ImmunoVision’s MPO is purified from a human promyelocytic cell line using detergent extraction, salt fractionation, and chromatographic techniques. Anti-neutrophil cytoplasmic antibodies (ANCA) are autoantibodies against cytoplasmic constituents of neutrophils and monocytes (2). Staining by indirect immunofluorescence (IFA) shows two main staining patterns: cytoplasmic (c-ANCA) and perinuclear (p-ANCA) (3,4). Anti-MPO antibodies usually generate a p-ANCA pattern but not all p-ANCA patterns are caused by anti-MPO antibodies. Anti-MPO autoantibodies are strongly associated with idiopathic microscopic polyangiitis, crescentic glomeronephritis, polyarteritis nodosa, and Churg-Strauss syndrome (5,6). In addition, MPO antibody titer may correlate with disease activity (3).

Product Code:

CodeAmountPriceInquiry
MPO-3000
1mg
$750
MPO-3200
200 micrograms
$175

References:

  1. Hope HR, Remsen EE, Lewis, Jr. C, Heuvelman DM, Walker MC, Jennings M, Connolly DT. Protein Expression and Purification Vol 18:pg 269-276 (2000).
  2. Savige J et al. Immunopathology Vol 111: pg 507-513 (1999).
  3. Peter JB. Use and Interpretation of Tests in Clinical Immunology, Eight Edition. Specialty Laboratories, Inc. Santa Monica, CA. pg 174-177.
  4. Hagen EC, et al. J.Immunol. Methods. Vol 196:pg 1-15 (1996).
  5. Cohen-Tervaert JW, et al. Arthritis Rheum. Vol 33(8):pg 1264-1272 (1990).
  6. Falk RJ, Jenette JC. N Engl J Med. Vol 318:pg 1651-1657 (1988).

Specifications:  

  • MPO-3000

Certificate of Analysis: 

  • 5375 

  • 5376 

  • 5243

  • 5839

  • 5855

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