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Clickchemistrytools/MB 543 DBCO/1174-1/1 mg

MB™543DBCOisanorangecoloredfluorescentprobewithabsorptionandemissionmaximaat544and560nm,respectively.Thedyeisbasedonanovelclassofrhodaminedyes.UnliketrADItionalrhodaminedyes,MB™543isnotonlybrightandphotostablebutalsohighlywater-solubleandpH-insensitive.MB™543fluorescentdyescanbeusedinplaceofTAMRA,AlexaFluor546®,DyLight546®,CF™543andspectrallysimilardyes.

MB™543DBCOdyereactswithazidesviaacopper-free“Clickchemistry”reactiontoformastabletriazoleanddoesnotrequireCu-catalystorelevatedtemperatures.InapplicationwherethepresenceofcopperisaconcernMB™543DBCOisanidealalternativetocopperrequiringfluorescentalkynes.

MB™543DBCOreagentisnotsuitableforstainingintracellularcomponentsoffixedandpermeABIlizedcellsduetohighbackgrounds.

 

Abs/EmMaxima543/563
Extinctioncoefficient105,000
Flowcytometrylaserline532nm
Microscopylaserline543or546nm
SpectrallysimilardyesAlexaFluor®546,Atto™543,CF™555Dye,DyLight™549
Molecularweightca.1080
CASN/A
SolubilityWater,DMSO,DMF,MeOH
Purity>95%(HPLC)
AppearanceRedsolid
Storageconditions-20C
ShippingconditionsAmbienttemperature
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