Click Chemistry is a new approach to rapidly and selectively react ("click") a pair of functional groups with each other in mild, aqueous conditions. Click Chemistry reactions can be categorized into two separate groups: (1) Cu(I)-catalyzed Azide-Alkyne Click Chemistry reaction (CuAAC); (2) Strain-promoted Azide-Alkyne Click Chemistry reaction (SPAAC).
Biomolecules that have been functionalized with a CLICK moiety (e.g. a terminal Alkyne or Azide) can be detected by labeling with a compatible CLICKable fluorescent dye (e.g. a fluorescent Azide or Alkyne) that allows the subsequent visualization by fluorescence spectroscopy. GeneCopoeia has developed a series of CLICKable biotin and fluorescent dyes for efficient “click” labeling of biomolecules.