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Clickchemistrytools/5-TAMRA Azide/1245-1/1 mg

5-TAMRAAzide(alsoknownasTAMRAazide,isomer5)isthered-fluorescentprobethatiscompatIBLewithvariousexcitationsourcesincludingmercuryarc,tungstenandxenonarclamps,the544nmlineoftheHelium-Neonlaserandthe532nmgreenlaserline.Itispredominantlyusedfordetectionofterminalalkyne-taggedbiomoleculesviaacopper-catalyzedclickreaction(CUAAC).Italsoreactswithstrainedcyclooctyneviaacopper-free“Clickchemistry”reactiontoformastabletriazoleanddoesnotrequireCu-catalystorelevatedtemperatures.

Althoughthemixedisomersof5(6)-TAMRAazideisapreferred,routinelyusedredfluorescentdyeforstainingproteins,labelingpeptidesandnucleotideswithTAMRAmixedisomersmightbetroublesomeduetosignificantsignalbroadeninginHPLCpurification.PeptidesandnucleotideslabeledwithasingleisomerTAMRAusuallygivebetterresolutioninHPLCpurificationthatisoftenrequiredintheconjugationprocesses.

5-TAMRAazideisexactreplacementofInvitrogen’s(ThermoFisherScientific)Tetramethylrhodamine(TAMRA)Azide(Tetramethylrhodamine5-Carboxamido-(6-Azidohexanyl)),5-isomer(Catalognumber:T10182).
 

Abs/EmMaxima553/575
Extinctioncoefficient92,000
Flowcytometrylaserline532or555nm
Microscopylaserline532or555nm
SpectrallysimilardyesAlexaFluor®546,Atto™543,CF™555Dye,DyLight™549
Molecularweight555.67
CAS1006592-61-5
SolubilityWater,DMSO,DMF,MeOH
Purity>98%(HPLC)
AppearanceRedsolid
Storageconditions-20C
ShippingconditionsAmbienttemperature
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