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Water-solubleCy®5Maleimideisthemostcommonlyusedsulfhydryl-reactivefluorescentlabelingreagentsforpreparingfar-red-fluorescentbioconjugates.Cy5®conjugatesareverybright,photostable,pHinsensitiveandproduceminimalautofluorescenceofbiologicalspecimensinthisregionofthespectrum.Water-solubleCy5®dyecanbeusedinplaceofAlexaFluor647®,DyLight649®andotherdyesexcitableatawavelengthof633or647nm.
Thissulfonateddyeisalsoknownassulfo-Cyanine5Maleimide.Cy®DyeisatrademarkofGEHealthcare.
| Abs/EmMaxima | 649/670nm |
| Extinctioncoefficient | 250,000 |
| Flowcytometrylaserline | 633or635nm |
| Microscopylaserline | 633or635nm |
| Spectrallysimilardyes | AlexaFluor®647,Atto™647,CF™647Dye,DyLight™649 |
| Molecularweight | 786.89 |
| CAS | N/A |
| Solubility | Water,DMSO,DMF,MeOH |
| Purity | >95%(HPLC) |
| Appearance | Bluesolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |