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MB™532PicolylAzideisanadvancedfluorescentprobethatincorporatesacopper-chelatingmotiftoraisetheeffectiveconcentrationofCu(I)atthereactionsite.Thechelatingeffectofpicolylazidemoietytoraisetheeffectiveconcentrationofcopperatthereactionsiteissogreatthatitleadstoanincreaseofsignalintensityofupto40-fold.
Inaddition,theuseofMB™532PicolylAzideinsteadofconventionalMB™532Azideallowsforatleastatenfoldreductionintheconcentrationofthecoppercatalystwithoutsacrificingtheefficiencyoflabeling.
Insummary,theintroductionofapicolylmoietyintoanazideprobeleadstoasubstantialincreaseinthesensitivityofalkynedetection.Thiswillbeofspecialvalueforthedetectionoflowabundancetargetsorwheresignificantincreaseinsignalintensityisdesired.
MB™532PicolylAzideisabright,greenfluorescentprobewithexcitationideallysuitedforthe532nmlaserline.MB™532isbright,exceedinglyphotostableandpH-insensitivefluorescentdyethatcanbeusedinplaceofFluorescein,AlexaFluor532®,DyLight532®andotherdyesexcitableatawavelengthof532nm.
Learnmoreaboutpicolylazidereagents.
| Abs/EmMaxima | 532/554nm |
| Extinctioncoefficient | 78,000 |
| Flowcytometrylaserline | 532nm |
| Microscopylaserline | 532nm |
| Spectrallysimilardyes | AlexaFluor®532,Atto™532,CF™532dyes,DyLight™532 |
| Molecularweight | ca.810 |
| CAS | N/A |
| Solubility | DMSO,DMF,MeOH |
| Purity | >95% |
| Appearance | Redtoorangesolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |