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Desthiobiotinisasingle-ring,sulfur-freeanalogofbiotinthatbindstostreptavidinwithnearlyequalspecificitybutlessaffinitythanbiotin(Kd=1011vs.1015M,respectively).Consequently,desthiobiotinylatedproteinscanbeelutedreADIlyandspecificallyfromstreptavidinaffinityresinusingmildconditionsbasedoncompetitivedisplacementwithfreebiotin.Forpull-downexperimentswithBIOLOGicalsamples,thissoft-releasecharacteristicofdesthiobiotinalsohelpstominimizeco-purificationofendogenousbiotinylatedmolecules,whichremainboundtostreptavidinuponelutionofthetargetproteincomplexeswithfreebiotin.
| Molecularweight | 427.23 |
| Chemicalcomposition | C21H37N3O6 |
| CAS | N/A |
| Solubility | DMSO,DMF,DCM,THF,Chloroform |
| Purity | >90% |
| Appearance | Colorlesstoslightlyyellowoil |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |