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MB™543PicolylAzideisanadvancedfluorescentprobethatincorporatesacopper-chelatingmotiftoraisetheeffectiveconcentrationofCu(I)atthereactionsite.Thechelatingeffectofpicolylazidemoietytoraisetheeffectiveconcentrationofcopperatthereactionsiteissogreatthatitleadstoanincreaseofsignalintensityofupto40-fold.
Inaddition,theuseofMB™543PicolylAzideinsteadofconventionalMB™543Azideallowsforatleastatenfoldreductionintheconcentrationofthecoppercatalystwithoutsacrificingtheefficiencyoflabeling.
Insummary,theintroductionofapicolylmoietyintoanazideprobeleadstoasubstantialincreaseinthesensitivityofalkynedetection.Thiswillbeofspecialvalueforthedetectionoflowabundancetargetsorwheresignificantincreaseinsignalintensityisdesired.
MB™543Dyeisanorangecoloredfluorescentprobewithabsorptionandemissionmaximaat544and560nm,respectively.Thedyeisbasedonanovelclassofrhodaminedyes.UnliketrADItionalrhodaminedyes,MB™543isnotonlybrightandphotostablebutalsohighlywater-solubleandpH-insensitive.MB™543fluorescentdyescanbeusedinplaceofTAMRA,AlexaFluor546®,DyLight546®,CF™543andspectrallysimilardyes.
Learnmoreaboutpicolylazidereagents.
| Abs/EmMaxima | 543/563 |
| Extinctioncoefficient | 105,000 |
| Flowcytometrylaserline | 532nm |
| Microscopylaserline | 543or546nm |
| Spectrallysimilardyes | AlexaFluor®546,Atto™543,CF™555Dye,DyLight™549 |
| Molecularweight | ca.1020 |
| CAS | N/A |
| Solubility | Water,DMSO,DMF,MeOH |
| Purity | >95%(HPLC) |
| Appearance | Redsolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |