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Desthiobiotinisasingle-ring,sulfur-freeanalogofbiotinthatbindstostreptavidinwithnearlyequalspecificitybutlessaffinitythanbiotin(Kd=1011vs.1015M,respectively).Consequently,desthiobiotinylatedproteinscanbeelutedreadilyandspecificallyfromstreptavidinaffinityresinusingmildconditionsbasedoncompetitivedisplacementwithfreebiotin.Forpull-downexperimentswithbiologicalsamples,thissoft-releasecharacteristicofdesthiobiotinalsohelpstominimizeco-purificationofendogenousbiotinylatedmolecules,whichremainboundtostreptavidinuponelutionofthetargetproteincomplexeswithfreebiotin.
| Molecularweight | 1145.33 |
| Chemicalcomposition | C57H82N11O14 |
| CAS | N/A |
| Solubility | DMSO,DMF,DCM,THF,Chloroform |
| Purity | >95% |
| Appearance | Redamorphoussolidtoredoil |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |