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Desthiobiotinisasingle-ring,sulfur-freeanalogofbiotinthatbindstostreptavidinwithnearlyequalspecificitybutlessaffinitythanbiotin(Kd=1011vs.1015M,respectively).Consequently,desthiobiotinylatedproteinscanbeelutedreadilyandspecificallyfromstreptavidinaffinityresinusingmildconditionsbasedoncompetitivedisplacementwithfreebiotin.Forpull-downexperimentswithbiologicalsamples,thissoft-releasecharacteristicofdesthiobiotinalsohelpstominimizeco-purificationofendogenousbiotinylatedmolecules,whichremainboundtostreptavidinuponelutionofthetargetproteincomplexeswithfreebiotin.
| Molecularweight | 414.50 |
| Chemicalcomposition | C18H34N6O5 |
| CAS | 1306615-47-3 |
| Solubility | DMSO,DMF |
| Purity | >95% |
| Appearance | Colorlesstoslightlyyellowoil |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |