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Cy®3isabright,water-soluble,andpHinsensitiveorange-fluorescentdyethatcanbeexcitedusingthe532nmor555nmlaserlineandvisualizedwithTRITC(tetramethylrhodamine)filtersets.Cy3conjugatesgivelessbackgroundthanTAMRAandmostothercommonlyusedfluorescentdyes.
ThemaleimidederivativeofCy®3dyeisthemostpopulartoolforconjugatingthedyetoathiolgrouponantibodies,cysteine-containingpeptidesandotherthiol-containingmolecules.Themaleimidegroupspecificallyandefficientlyreactswithreducedthiols(sulfhydrylgroups,–SH)atpH6.5to7.5toformstablethioetherbonds.
Thissulfonateddyeisalsoknownassulfo-Cyanine3Maleimide.Cy®DyeisatrademarkofGEHealthcare.
| Abs/EmMaxima | 550/570nm |
| Extinctioncoefficient | 150,000 |
| Flowcytometrylaserline | 532555or568nm |
| Microscopylaserline | 532or555nm |
| Spectrallysimilardyes | TAMRA,AlexaFluor®555,Atto™555,CF™555Dye,DyLight™549 |
| Molecularweight | 760.85 |
| CAS | N/A |
| Solubility | Water,DMSO,DMF,MeOH |
| Purity | >95%(HPLC) |
| Appearance | Darkredsolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |