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TheNIR-fluorescentCy5azidecanbereactedwithterminalalkynesviaacopper-catalyzedclickreaction(CuAAC).Italsoreactswithstrainedcyclooctyneviaacopper-free“clickchemistry”reactiontoformastabletriazoleanddoesnotrequireCu-catalystorelevatedtemperatures.Cy5isawater-soluble,brightfluorescentlabelthatcanbeusedinplaceofAlexaFluor647®,DyLight649®andotherdyesexcitableatawavelengthof633or647nm.
Forthedetectionoflowabundancetargetsorwheresignificantincreaseinsignalintensityisdesiredpleaseconsiderusingnextgenerationazidesprobescontaininganinternalcopper-chelatingmotif.
Learmmoreaboutnextgenerationazidesprobes
Thissulfonateddyeisalsoknownassulfo-Cyanine5Azide.Cy®DyeisatrademarkofGEHealthcare.
| Abs/EmMaxima | 647/663nm |
| Extinctioncoefficient | 250,000 |
| Flowcytometrylaserline | 633or635nm |
| Microscopylaserline | 633or635nm |
| Spectrallysimilardyes | AlexaFluor®647,Atto™647,CF™647Dye,DyLight™649 |
| Molecularweight | 834.01 |
| CAS | 1782950-80-4 |
| Solubility | Water,DMSO,DMF |
| Purity | >95%(HPLC) |
| Appearance | Bluesolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |