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Cy5DBCOdyereactswithazidesviaacopper-free“clickchemistry”reactiontoformastabletriazoleanddoesnotrequireCu-catalystorelevatedtemperatures.InapplicationwherethepresenceofcopperisaconcernCy5DBCOisanidealalternativetocopperrequiringfluorescentalkynes.
Cy5DBCOreagentisnotsuitableforstainingintracellularcomponentsoffixedandpermeabilizedcellsduetohighbackgrounds.
| Abs/EmMaxima | 649/670nm |
| Extinctioncoefficient | 250,000 |
| Flowcytometrylaserline | 633or635nm |
| Microscopylaserline | 633or635nm |
| Spectrallysimilardyes | AlexaFluor®647,Atto™647,CF™647Dye,DyLight™649 |
| Molecularweight | 1009.22 |
| CAS | 1564286-24-3 |
| Solubility | Water,DMSO,DMF |
| Purity | >95%(HPLC) |
| Appearance | Bluesolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |