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TheNIR-fluorescentCy5.5azidecanbereactedwithterminalalkynesviaacopper-catalyzedclickreaction(CuAAC).Italsoreactswithstrainedcyclooctyneviaacopper-free“clickchemistry”reactiontoformastabletriazoleanddoesnotrequireCu-catalystorelevatedtemperatures.Cy5.5isawater-soluble,pH-insensitive,brightfluorescentprobethatcanbeusedinplaceofAlexaFluor®680,DyLigh®t680,orIRDye™680andotherdyesexcitableatawavelengthataround680nm.
Thissulfonateddyeisalsoknownassulfo-Cyanine5.5Azide.Cy®DyeisatrademarkofGEHealthcare.
| Abs/EmMaxima | 678/694nm |
| Extinctioncoefficient | 190,000 |
| Spectrallysimilardyes | AlexaFluor®680,IRDye®680RD,CF™680Dye,DyLight™680 |
| Molecularweight | 999.15 |
| CAS | N/A |
| Solubility | Water,DMSO,DMF |
| Purity | >95%(HPLC) |
| Appearance | Bluesolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |