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| AlternativeName: | ERα |
| Clone: | 33 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Syntheticpeptidecorrespondingtoaa247-261(E247VGMMKGGIRKDRRG261)oftheDNAbindingdomainofERα(estrogenreceptorα).Thissequenceiscompletelyconservedinmanyspeciesincludingmouse,rat,pig,bovine,sheep,chickenandfrog. |
| UniProtID: | P03372 |
| Speciesreactivity: | Human,Mouse,Rat Bovine,Monkey |
| Specificity: | RecognizesboththesteroidoccupiedandunoccupiedformsofERα. |
| Crossreactivity: | Doesnotcross-reactwithandrogenreceptor,PR(progesteronereceptor),GR(glucocorticoidreceptor)orERβ. |
| Applications: | FlowCytometry,ICC,IHC(FS),IHC(PS),IP,WB |
| RecommendedDilutions/Conditions: | Immunocytochemistry(10µg/ml) Immunohistochemistry(frozensectionsandparaffinsections,5µg/ml) WesternBlot(1µg/ml) Suggesteddilutions/conditionsmaynotbeavailableforallapplications. Optimalconditionsmustbedeterminedindividuallyforeachapplication. |
| ApplicationNotes: | Immunohistochemistry:StainingofERαinmouseLeydigcellsresultsinbothnuclearandcytoplasmicstaining. Detectsabandof~67kDabyWesternblot. DoesnotinhibitbindingofERtonucleiandDNA. |
| Formulation: | Liquid. InPBS containing0.1%sodiumazide. |
| Handling: | Avoidfreeze/thawcycles. |
| Shipping: | ShippedonBlueIce |
| LongTermStorage: | -20°C |
| ScientificBackground: | Steroidreceptorsarealargesuperfamilyofligand-dependentintracellularproteinsthatstimulatetranscriptionofspecificgenesbybindingtospecificDNAsequencesfollowingactivationbytheappropriatehormone.Estrogenreceptor(ER)hasbeenclassifiedintotwodistinctisoforms,αandβ.ERαisknowntointeractinaliganddependentandindependentmannerwithseveralknowncoactivatorsincludingReceptor-InteractingProtein140(RIP140/ERAP140),ERAP160,andSteroidReceptorCoactivator-1(SRC-1)toenhancetranscriptionalactivityoftargetgenes. |
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