R&D Systems/Human/Mouse/Rat GSK-3 beta Antibody/MAB2506/100 ug

• Species Reactivity
  Human, Mouse, Rat
• Specificity
  Detects human, mouse, and rat GSK-3 beta  in ELISAs and Western blots. Does not cross-react with recombinant human GSK-3 alpha.
• Source
  Monoclonal Rat IgG_2B Clone # 272536
• Purification
  Protein A or G purified from hybridoma culture supernatant
• Immunogen
  E. coli-derived recombinant human GSK-3 beta
  Met1-Thr420
  Accession # P49841
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  1 µg/mL
  See below
• CyTOF-ready
  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
• Intracellular Staining by Flow Cytometry
  2.5 µg/10⁶ cells
  HeLa human cervical epithelial carcinoma cell line fixed with paraformaldehyde and permeabilized with methanol.
  

• Reconstitution
  Reconstitute at 0.5 mg/mL in sterile PBS.
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• Long Name:
  Glycogen Synthase Kinase 3 beta
• Entrez Gene IDs:
  2932 (Human); 56637 (Mouse); 84027 (Rat)
• Alternate Names:
  EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK3 beta; GSK-3 beta; GSK3B; GSK3beta isoform

Background: