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The Malachite Green Phosphate Detection Kit is a convenient and sensitive, single-step free-phosphate determination kit that can be used for measuring phosphate released during enzymatic phosphatase assays.
The Malachite Green Phosphate Detection Kit detects only free-phosphate in sample. Figure 1 demonstrates a linear range of 50 to 1600 pmol of phosphate.
The Malachite Green Phosphate Detection Kit is designed for the in vitro measurement of phosphatase activity. Protein phosphatase enzymatic activity results in the release into solution of free inorganic phosphate from protein-phosphatase substrates. This free inorganic phosphate then forms a green molybdophosphoric acid complex. Formation of this complex is monitored and quantified by measuring the absorbance at 630 nm. This absorbance is proportional to free-inorganic phosphate in solution and correlates with the activity of phosphatase (1,2).
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