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DiscoverX/PathHunter® Aflibercept Bioassay Kit/200dp(2x96-well)/93-0996Y1-00005

The PathHunter® Dimerization Bioassay detects ligand induced dimerization of two subunits of a receptor-dimer pair.The cells have been engineered to co-express one receptor subunit fused to Enzyme Donor (ED), and a second dimer partner fused to Enzyme Acceptor (EA).Cytoplasmic tail may have been deleted from one or both receptors.Binding of an agonist to one receptor subunit induces it to interact with its dimer partner,forcing complementation of the two enzyme fragments.This results in the formation of a functional enzyme that hydrolyzes a substrate to generate a chemiluminescent signal.
Target/Description
Kinase Insert Domain Receptor
Group
Receptor Tyrosine Kinase
Cell Background
HEK 293
Species
Human
Accession Number
NM_002253.2
Agonist/Ligand
VEGF-165
Assay Measures
Dimerization
Bioassay Target Name
KDR/KDR
Assay Qualified With
Eylea®
Drug Trademark Owner
Eylea®is a registered trademark of Regeneron Pharmaceuticals, Inc.
How it works
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