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EnzScript™ (M-MLV Reverse Transcriptase RNase H minus) is an RNA-dependent DNA polymerase with no detectable RNase H activity. EnzScript™ can be used to generate first-strand cDNA from polyA mRNA or total RNA for use in downstream applications such as RT- PCR, cDNA cloning or library construction for RNA-Seq. Point mutations in the RNase H domain increase the thermostability of the enzyme and support greater cDNA yield of full-length transcripts than wild type M-MLV Reverse Transcriptase (1).
Source of Protein: A recombinant E. coli strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene with 3 point mutations in the RNase H domain that eliminate detectable RNase H activity.
Unit Definition: 1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.
Molecular weight: 75,938 Daltons
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