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Lambda Exonuclease is a highly processive 5′→3′ double-stranded exonuclease that degrades one strand of the duplex. Lambda exonuclease can initiate at blunt DNA or DNA containing 3′ single-stranded overhangs. Lambda Exonuclease has greatly reduced activity on non-phosphorylated DNA, single-stranded DNA and DNA having protruding 5′ single-stranded termini. Lambda exonuclease will not initiate at a nick or gap.
Source of ProteinPurified from a strain of E. coli that overexpresses the exonuclease gene from bacteriophage Lambda.
Supplied in25 mM Tris-HCl50 mM NaCl1.0 mM DTT0.1 mM EDTA50% GlycerolpH 7.5 @ 25°C
Supplied WithB8030 (10X Lambda Exo Reaction Buffer)
10X Lambda Exo Reaction Buffer (B8030)670 mM Glycine25 mM MgCl2pH 9.4 @ 25°C
Unit DefinitionOne unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in 30 minutes at 37°C.
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