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T7 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′ phosphate and a 3′ hydroxyl termini in duplex DNA. The enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA.
Source of ProteinA recombinant E. coli strain carrying the T7 DNA Ligase gene.
Supplied in20 mM Tris-HCl50 mM NaCl1 mM DTT0.1 mM EDTA50% glycerolpH 7.5 @ 25°C
Supplied With:B1010 (2X Rapid Ligation Buffer)
2X Rapid Ligation Buffer (B1010):132mM Tris-HCI20 mM MgCl22 mM DTT2 mM ATP15% PEG 6000pH 7.6 @ 25°C
Unit Definition 1 unit is defined as the amount of T7 DNA Ligase required to ligate 50% of 100 ng DNA fragments with cohesive termini in 30 minutes at 23°C.
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