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T4 DNA Polymerase catalyzes the extension of a primed DNA template in the 5′→ 3′ direction. This enzyme exhibits a powerful 3′→ 5′ exonuclease activity, while lacking any inherent 5′→ 3′ exonuclease or strand displacement functions.
Source of ProteinPurified from a strain of E. coli that expresses the recombinant T4 DNA Polymerase gene.
Supplied in100 mM KPO41.0 mM DTT0.1 mM EDTA50% glycerolpH 6.5 @ 25°C
Supplied WithB0110 (10X Blue Buffer)
10X Blue Buffer (B0110)500 mM NaCl100 mM Tris-HCl100 mM MgCl210 mM DTTpH 7.9 @ 25°C
Unit DefinitionOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-precipitable material in 30 minutes at 37°C.
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