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Epigentek/EpiQuik HDAC1 Assay Kit (Colorimetric)/P-4005-96/96 assays

InputType:NuclearExtracts,PurifiedEnzyme
ResearchArea:HistoneAcetylation
TargetApplication:AmountQuantitation
VesselFormat:96-WellPlate
100%Guarantee:6months
ProductOverview

TheEpiQuik™HDAC1AssayKitisaconvenientpackageoftoolsthatallowstheexperimentertomeasuretheamountofHDAC1attremendouslyfastspeedsandconsistency;superiorandsaferthanallothercurrentmethods.Thekitisready-to-useandprovidesalltheessentialcomponentsneededtocarryoutasuccessfulHDAC1assayexperimentwithouttheneedforelectrophoresisandtransferprocess.ItissuitableformeasuringHDAC1levelfromvariousfreshtissuesandculturedmammaliancells.Thekithasthefollowingadvantages:

  • Fastprocedure,whichcanbefinishedwithin3hours.
  • Innovativecolorimetricassaytosemi-quantitativelymeasureHDAC1amountwithouttheneedforelectrophoresis.
  • StripmicroplateformatmakestheassayflexIBLe:manualorhighthroughputanalysis.
  • Simple,reliable,andconsistentassayconditions.

Principle&Procedure
TheEpiQuik™HDAC1AssayKitisdesignedformeasuringtotalHDAC1amountfromtissuesorcells.Inanassaywiththiskit,thenuclearproteinscontainingHDAC1arestablycoatedonthestripwells.TheHDAC1isrecognizedwithahigh-affinityspecificantibody.TheamountofHDAC1canbequantifiedthroughaHRPconjugatedsecondaryantibody-colordevelopmentsystemandisproportionaltotheintensityofcolordevelopment.

 
Fig.1.SchematicprocedureforusingtheEpiQuikHDAC1AssayKit(Colorimetric).

Fig.2. HDAC1proteinswereboundtomicroplatewellsatdifferentconcentrationsandmeasuredusingtheEpiQuik™HDAC1AssayKit.

Fig.3. Nuclearproteinswerepreparedfromvarioushumantissuesamples.5µgofnuclearproteinswereboundtomicroplatewellsforHDAC1levelmeasurementusing theEpiQuik™HDAC1AssayKit.

ProductComponents

HA1(10XWashBuffer)
HA2(HDACAssayBuffer)
HA3(BlockingBuffer)
HA4(CaptureAntibody,200µg/ml)*
HA5(DetectionAntibody,200µg/ml)*
HA6(DevelopingSolution)
HA7(StopSolution)
HDAC1Control(100ng/µl)*
8-WellAssayStrip(withFrame)
UserGuide

*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialafterthawingpriortoopeningthecap.

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