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Stemcell/Anti-Human CD14 Antibody, Clone M5E2/25 Tests/60004FI.1188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Stemcell/Anti-Human CD14 Antibody, Clone M5E2/25 Tests/60004FI.1

Overview

TheM5E2antibodyreactswithCD14,an~53-55kDaGPI-anchoredtransmembraneglycoproteinexpressedathighlevelsonthesurfaceofperipheralbloodmonocytesandmacrophages,andatlowerlevelsongranulocytes.An~10-folddifferenceinexpressionlevelsbetweenmonocytes/macrophagesandgranulocytesmakesCD14ausefulMarkerfordistinguishingthesecellpopulations.CD14isalsofoundontissuemacrophages,Langerhanscellsanddendriticcells.CD14functionsasahigh-affinityreceptorforcomplexesoflipopolysaccharide(LPS)andserumLPS-bindingproteinandmodulatesLPS-dependentsignaltransductionduringtheimmuneresponsetogram-negativepathogensbyactingasaco-receptorforTLR4andMD-2.ThistriggersactivationofNF-kappa-B,cytokinesecretion,andinductionoftheinflammatoryresponse.TwosolubleformsofCD14havealsobeendescribed(~48and~55kDa).ThisantibodyclonehasbeenverifiedforpurityassessmentsofcellsisolatedwithEasySep™kits,includingEasySep™HumanMyeloidPositiveSelectionKit(Catalog#18653)andEasySep™HumanCD14PositiveSelectionKit(Catalog#18058);partialblockingmaybeobserved,aswellasEasySep™HLAWholeBloodCD33PositiveSelectionKit(Catalog#18287HLA),EasySep™HLAWholeBloodMyeloidPositiveSelectionKit(Catalog#18683HLA),andEasySep™HumanBuffyCoatCD14PositiveSelectionKit(Catalog#18088).
Subtype:
PrimaryAntibodies
TargetAntigen:
CD14
AlternativeNames:
LPSreceptor
ReactiveSpecies:
CapuchinMonkey;CommonMarmoset;Cotton-ToppedTamarin;Cynomolgus;Rhesus;SquirrelMonkey;Chimpanzee;Cow;Dog;Human;Macaque;Pig;Sheep
Conjugation:
AlexaFluor488;APC;Biotin;FITC;PE;Unconjugated
HostSpecies:
Mouse
CellType:
Monocytes
Application:
CyTOF;FlowCytometry;FunctionalAssay;Immunocytochemistry;Immunofluorescence;Immunohistochemistry;NeutralizationandBlocking
AreaofInterest:
Immunology
Clone:
M5E2
GeneID:
929
Isotype:
IgG2a,kappa
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