Description:RecombinantmonoclonalantibodytoVP1.ManufacturedusingAbAb’sRecombinantPlatformwithvariableregions(i.e.specificity)fromthehybridoma4C4.ThisantibodywascreatedusingourproprietaryFcSilent™engineeredFcdomaincontainingkeypointmutationsthatabrogatebindingtoFcgammareceptors.
ThischimerichumanantibodywasmadeusingthevariabledomainsequencesoftheoriginalMouseIgG2aformat,forimprovedcompatibilitywithexistingreagents,assaysandtechniques.
SpeciesandIsotype:HumanIgG1,FcSilent™,kappa
CloneNumber:4C4
UniProtAccessionNumberofTargetProtein:PublishedApplication(s):NTRL;crystallisation;
ELISA;WB
PublishedSpeciesReactivity:Foot-and-mouthdiseasevirus
Immunogen:MurineantibodieswereraisedagainstthetypeC1BresciaIt/64strainofFMDV.
Specificity:4C4specificallybindstheG-Hloop,alsoknownasantigenicsiteA,oftypeCFMDVcapsidproteinVP1.Thisspecificityissupportedbyplaquereductionneutralisationassays(Verdagueretal,1999)aswellasmorerecentcrystallographicdata(Verdagueretal,1998;Ochoaetal,2000).VP1isacoatproteinrequiredforhostcellrecognitionandmayfacilitateFMDVentryduringinfection.Foot-and-mouthdiseaseaffectsbovinespecies,andinsomecasescanbelethal.
ApplicationNotes:4C4possessesstrongneutralisationactivityagainsttheVP1G-Hloop,orantigenicsiteA,of
SEROtypeCFMDV.Thishasbeenshownusingplaquereduction(neutralisation)assaysaswellasELISAsandimmunoblotting(Mateuetal,1992,1989,1990).
AntibodyFirstPublishedin:Mateuetal.Asingleaminoacidsubstitutionaffectsmultipleoverlappingepitopesinthemajorantigenicsiteoffoot-and-mouthdiseasevirusofserotypeCJournalofGeneralVirology(1990),71,629-637.
Noteonpublication:DescribestheneutralisationactivityofmAbsagainstFMDVserotypeCstrains,andthemappingofmAbstoviralepitopesusingsyntheticpeptidescorrespondingtoregionsofvirusproteins.
ProductForm
Purification:ProteinAaffinitypurified
Suppliedin:PBSwithpreservative(0.02%Proclin300)
StorageRecommendation:Storeat4°Cforupto3months.Forlongertermstoragealiquotintosmallvolumesandstoreat-20°C
Concentration:Seeviallabel.
Importantnote–Thisproductisforresearchuseonly.Itisnotintendedforuseintherapeuticordiagnosticproceduresforhumansoranimals.
