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Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). CCL19 is expressed abundantly in thymus and lymph nodes, with moderate levels in trachea and colon and low levels in stomach, small intestine, lung, kidney and spleen. The gene for CCL19 is located on human chromosome 9. This chemokine elicits its effects on its target cells by binding to the chemokine receptor CCR7. It attracts certain cells of the immune system, including dendritic cells and antigen-engaged B cells.
Macrophage Inflammatory Protein-3 beta human recombinant (CCL19) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8809 Dalton. The MIP-3b is purified by proprietary chromatographic techniques. Purity is greater than 98.0% as determined by RP-HPLC and SDS-PAGE.
The sequence of the first five N-terminal amino acids was determined and was found to be, Gly-Thr-Asn-Asp-Ala.
The activity of CCL19 is calculated by the ability to chemoattract human T cells using a concentration of 10-50 ng/ml corresponding to a specific activity of 20,000-100,000 IU/mg.
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