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Biognosys/Sample Preparation Kit/1kit/Ki-3010

Sample Preparation

Sample Preparation Kit

Simple-to-use standardized sample preparation kit for reproducible mass spectrometry proteomics

The protocols work well with all mass spectrometry based proteomics applications such as SRM/MRM, HRM/DIA/SWATH and shotgun MS/MS.

Product number: Ki-3010

USD

300.00

In stock

Variations in sample preparation between experiments and individual samples are an enormous source of experimental bias in proteomics, especially in high-throughput applications. Since the proteomes of natural samples are extremely complex with typically 100"000s of peptides present, spanning over several orders of magnitude, it is challenging to establish a reliable method for preparing protein samples for analysis by mass spectrometry.

Biognosys’ Sample Preparation Kit is a simple-to-use standardized kit for reproducible mass spectrometry proteomics of mammalian cell cultures and plasma/serum samples. The protocols work well with all mass spectrometry based proteomics applications such as SRM/MRM, HRM/DIA/SWATH and shotgun MS/MS.

Please contact us for support.

Sample Preparation Kit components

  • Alkylation Solution (1x 10 ml tube)
  • Reduction Stock Solution (1x 0.5 ml tube)
  • LC Solution (1x 10 ml tube)
  • 10x Dilution Buffer (1x 10 ml tube)
  • Denature Buffer (1x 10 ml tube)

The kit components are sufficient for 50 plasma or 50 mammalian cell culture sample preparations to be made at once. It requires 10 µl per sample of plasma/serum or mammalian cell culture pellets with at least 100 µg of total protein per sample.

Please contact us for support.

Sample Preparation Kit Manual

Sample Preparation Kit Quick Reference Card

Sample Preparation Kit Safety Data Sheet (MSDS)

Extension protocol for frozen tissues

Please contact us for support.

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