Sample Preparation
The protocols work well with all mass spectrometry based proteomics applications such as SRM/MRM, HRM/DIA/SWATH and shotgun MS/MS.
Variations in sample preparation between experiments and individual samples are an enormous source of experimental bias in proteomics, especially in high-throughput applications. Since the proteomes of natural samples are extremely complex with typically 100"000s of peptides present, spanning over several orders of magnitude, it is challenging to establish a reliable method for preparing protein samples for analysis by mass spectrometry.
Biognosys’ Sample Preparation Kit is a simple-to-use standardized kit for reproducible mass spectrometry proteomics of mammalian cell cultures and plasma/serum samples. The protocols work well with all mass spectrometry based proteomics applications such as SRM/MRM, HRM/DIA/SWATH and shotgun MS/MS.
The kit components are sufficient for 50 plasma or 50 mammalian cell culture sample preparations to be made at once. It requires 10 µl per sample of plasma/serum or mammalian cell culture pellets with at least 100 µg of total protein per sample.
Sample Preparation Kit Manual
Sample Preparation Kit Quick Reference Card
Sample Preparation Kit Safety Data Sheet (MSDS)
Extension protocol for frozen tissues
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn