Cell Biologics/Mouse Primary Diabetic Pulmonary Vein Endothelial Cells/MD-6060/

MousePrimaryDiabeticPulmonaryVeinEndothelialCells

CatalogNo.  MD-6060

SuggestedMedium:EndothelialCellMedium/wKit–500ml

CatalogNo.  M1168

ProductDescription

MousePrimaryDiabeticPulmonaryVeinEndothelialCellsfrom CellBIOLOGics areisolatedfromthepulmonaryveinofDiabetic(db/db)mice.MousePrimaryDiabeticPulmonaryVeinEndothelialCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedsolutionfor2minandincubatedin CellBiologics’ CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsatpassage3aredetachedfromflasksandimmediatelycryopreservedinvials.Eachvialcontainsatleast0.5×10⁶ cellsperml.Themethodweusetoisolateprimaryendothelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithPECAM-1antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.

ProductTesting

MousePrimaryDiabeticPulmonaryVeinEndothelialCellsaretestedforexpressionofMarkersusingantibody,VE-cadherin(CD144,VE-cadherinAntibody,C-19,sc6458,SantaCruz);AF1002(R&DSystem)orCD31/PECAM-1(PurifiedRatAnti-MouseCD31,CatalogNo.553370,BD)byimmunofluorescencestainingorFACS.Cellsarenegativeforbacteria,yeast,fungiandmycoplasma.Cellscanbeexpandedfor3-7passagesatasplitratioof1:2underthecellcultureconditionsspecifiedby CellBiologics. Repeatedfreezingandthawingofcellsisnotrecommended.

LaboratoryApplications

MousePrimaryDiabeticPulmonaryVeinEndothelialCellscanbeusedinassaysofcelltocelladhesion,migration,vasculartubeformation.StandardbiochemicalproceduresperformedwithendothelialcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentstaining,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.

Storageof CellBiologics’ Products

CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.Primarycellsshouldneverbekeptat-20°C.

AuthorizedUsesof CellBiologics’ Products

MousePrimaryDiabeticPulmonaryVeinEndothelialCellsfrom CellBiologics aredistributedforinternalresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.

Disclaimer

Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineat http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

WarrantyandLiABIlity

CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ Cellswith CellBiologics’ Media(catalognumberM1168)andCoatingSolutionforappropriateendothelialcellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.