Cell Biologics/Porcine Primary Kidney Fibroblasts/P-6016/ T75 Flask

PorcinePrimaryKidneyFibroblasts

CatalogNo. P-6016

SuggestedMedium: FibroblastMedium/wKit-500ml

CatalogNo. M2267

CustomOrder(1-3weeks)

ProductDescription

PorcinePrimaryKidneyFibroblastsfromCellBIOLOGicsareisolatedfromkidneytissueofporcine.PorcinePrimaryKidneyFibroblastsaregrowninT75tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologicsCultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded. Priortoshipping,cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x10⁶cellspermlandisdeliveredfrozen. 

ProductTesting

PorcinePrimaryKidneyFibroblastsaretestedfornegativeexpressionofvonWillebrandFactorExpression/FactorVIII,cytokeratin18,andalphasmoothmuscleactin.PorcinePrimaryKidneyFibroblastsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-5passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.

LaboratoryApplications

PorcinePrimaryKidneyFibroblastscanbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.

StorageofCellBiologicsProducts

CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest.

Nevercanprimarycellsbekeptat-20°C.

AuthorizedUsesofCellBiologicsProducts

PorcinePrimaryKidneyFibroblastsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.

Disclaimer

InvestigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.

WarrantyandLiABIlity

CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.